Recombinant Human GSTM1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6894-GT

R&D Systems, part of Bio-Techne
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6894-GT-010
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Key Product Details

Source

E. coli

Accession #

P09488

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Glutathione S-Transferase mu 1/GSTM1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Glutathione S-Transferase mu 1/GSTM1 protein
Met1-Lys218

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1 and Pro2

Predicted Molecular Mass

26 kDa

SDS-PAGE

25-27 kDa, reducing conditions

Activity

Measured by the conjugation of reduced glutathione to 1-bromo-2,4-dinitrobenzene.
The specific activity is >20,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6894-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Glutathione S-Transferase mu 1/GSTM1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. The gene encoding GSTM1 is mapped onto human chromosome 1p13.3 and is known to be highly polymorphic (1). Mostly notably, the widely occurring GSTM1-null genotype has been linked to a variety of cancers including lung (2), gastric (3), bladder (4) and prostate (5). In addition to its role in releasing oxidative stress, GSTM1 has also been suggested to act as a hormone binding protein and play a role in maintaining hormone homeostasis in the body (6, 7).

References

  1. Pearson, W. R. et al. (1993) Am. J. Hum. Genet. 53:220.
  2. Mohr, L.C. et al. (2003) Anticancer. Res. 23:2111.
  3. Wang, H. et al. (2010) Dig. Dis. Sci. 55:1824.
  4. Engel, L. S. et al. (2002) Am. J. Epidermiol. 156:95.
  5. Mo, Z. et al. (2009) Prostate. 69:662.
  6. Mukherjee, S. B. et al. (1999) Biochem. J. 340:309.
  7. Ishigaki, S. et al. (1989) Arch. Biochem. Biophys. 273:265.

Alternate Names

Glutathione STransferase mu 1, GST1, GSTM1, GTH4, GTM1

Entrez Gene IDs

2944 (Human)

Gene Symbol

GSTM1

UniProt

P09488

Additional Glutathione S-Transferase mu 1/GSTM1 Products

Product Documents for Recombinant Human GSTM1 Protein, CF

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Product Specific Notices for Recombinant Human GSTM1 Protein, CF

For research use only

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