Skip to main content

Recombinant Human Guanylate Kinase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9267-GU

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
9267-GU-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human Guanylate Kinase protein
Ser2-Ala197, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

23 kDa

SDS-PAGE

24 kDa, reducing conditions

Activity

Measured by its ability to transfer phosphate from ATP to GMP.
The specific activity is >7,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9267-GU
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Guanylate Kinase

GUK1, also known as GMP kinase, belongs to the guanylate kinase family. This protein exists as a monomer and catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP (1). Via its catalytic activity, GUK1 participates in regulating the supply of guanine nucleotides including cGMP to signal transduction pathways (2). GUK1 is widely expressed. Overexpression of GUK1 is associated in pituitary adenomas (3). The enzymatic activity of the recombinant human GUK1 is measured using a phosphatase-coupled method (4).

References

  1. Brady, W.A. et al. (1996) J. Bio.Chem. 271:16734.
  2. Fitzgibbon, J. et al. (1996) FEBS Letters 385:185.
  3. Da Rocha, A.A. et al. (2006) Pituitary 9:83.
  4. Wu, Z.L. (2011) PLoS ONE 6:e23172.

Alternate Names

ATP:GMP Phosphotransferase, Deoxyguanylate Kinase, GMK, GMP Kinase, Guanosine monophosphate Kinase, GUK1

Entrez Gene IDs

2987 (Human); 14923 (Mouse); 303179 (Rat)

Gene Symbol

GUK1

UniProt

Additional Guanylate Kinase Products

Product Documents for Recombinant Human Guanylate Kinase Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Guanylate Kinase Protein, CF

For research use only

Loading...
Loading...
Loading...