Recombinant Human Heparan Sulfate-6-O-Sulfotransferase-3, CF

R&D Systems, part of Bio-Techne | Catalog # 8568-ST

R&D Systems, part of Bio-Techne
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8568-ST-020
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Key Product Details

Source

CHO

Accession #

Q8IZP7

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 protein
Pro28-Trp471, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

52 kDa

SDS-PAGE

60-95 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to heparan sulfate.
The specific activity is >65 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8568-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3

Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and extracellular matrix. It is usually covalently attached to a protein core as the glycan component of a proteoglycan. Heparan sulfate interacts with a variety of proteins, such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, therefore playing roles in cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism and viral infection (1, 2). Heparan sulfate consists of repeating residues of uronic acid and Nacetylglucosamine. The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O-sulfotransferase. The N-acetylglucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases, heparan sulfate 3-O and 6-O sulfotransferases, respectively. However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of heparan sulfate. As a result, heparan sulfate displays enormous sequence diversity that allows it to interact with a wide spectrum of proteins differently. Three heparan sulfate 6-O-sulfotransferases are found both in human and mouse possibly with overlapping substrate specificity (3). HS6ST3 is ubiquitously expressed while HS6ST1 and HS6ST2 are expressed primarily in the liver and brain/spleen, respectively. The enzyme activity of the recombinant HS6ST3 is measured using a phosphatase-coupled assay (7).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J. D. and Selleck, S. B. (2002) Annu. Rev. Biochem. 71:435.
  3. Habuchi, H. et al. (2000) J. Biol. Chem. 275:2859.
  4. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  5. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
  6. Wu, Z.L., et al. (2002) Faseb J. 16:539.
  7. Prather, B. et al. (2012) Anal.Biochem.423:86

Alternate Names

DKFZp761K2315, heparan sulfate 6-O-sulfotransferase 3

Entrez Gene IDs

266722 (Human); 50787 (Mouse)

Gene Symbol

HS6ST3

UniProt

Q8IZP7

Additional Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Products

Product Documents for Recombinant Human Heparan Sulfate-6-O-Sulfotransferase-3, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Heparan Sulfate-6-O-Sulfotransferase-3, CF

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