Recombinant Human Hepassocin/FGL1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10285-HE

R&D Systems, part of Bio-Techne
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10285-HE-050
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Key Product Details

Source

NS0

Accession #

BAB70690.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Hepassocin/FGL1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Hepassocin/FGL1 protein
Leu23-Ile312, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

35 kDa

SDS-PAGE

33-37 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LAG-3 Fc Chimera (Catalog # 2319-L3) is coated at 1 μg/mL (100 μL/well), the concentration of Human Hepassocin/FGL1 His-tag (Catalog # 10285-HE) that produces 50% of the optimal binding response is 0.05-0.4 μg/mL.

Scientific Data Images for Recombinant Human Hepassocin/FGL1 His-tag Protein, CF

Recombinant Human Hepassocin/FGL1 His-tag Protein Binding Activity

Recombinant Human Hepassocin/FGL1 His-tag Protein Binding Activity

When Recombinant Human LAG-3 Fc Chimera (Catalog # 2319-L3) is coated at 1 µg/mL (100 µL/well), the concentration of Human Hepassocin/FGL1 His-tag (Catalog # 10285-HE) that produces 50% of the optimal binding response is 0.05-0.4 µg/mL.
Recombinant Human Hepassocin/FGL1 His-tag Protein SDS-PAGE

Recombinant Human Hepassocin/FGL1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Hepassocin/FGL1 His-tag (Catalog # 10285-HE) was resolved with SDS-PAGE under reducing (R) and non reducing (NR) conditions and visualized by Coomassie® Blue staining , showing single bands at 33-37 kDa and 65-75 kDa, respectively.

Formulation, Preparation and Storage

10285-HE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and Fibrinogen-Like Protein 1 (FGL1) (1), is a liver-specific secreted protein belonging to the fibrinogen superfamily, whose members share a fibrinogen domain at their C-termini (2). Human Hepassocin/FGL1 is a secreted homodimer consisting of 312 amino acids (aa) with a 22 aa signal sequence and a 290 aa mature protein (3). Mouse and rat Hepassocin/FGL1 share approximately 84% and 83% amino acid identity with human Hepassocin/FGL1, respectively. Human Hepassocin/FGL1 binds LAG-3 through its fibrinogen-like domain independently of MHC class II (4). Hepassocin/FGL1 inhibits antigen-specific T cell activation, with its elevated presence in plasma of cancer patients indicating poor prognoses (4). Other than this role in cancer progression, Hepassocin/FGL1 also has restorative function for liver cells, as it is upregulated during liver regeneration following partial hepatectomy (5), and stimulates proliferation of hepatocytes in vivo and improves prognoses with fulminant hepatic failure in rats (6). Its expression is regulated in Hep G2 cells by interleukin-6 (IL-6) and is found in the serum in both bound and unbound states as an acute phase reactant (7).

References

  1. Yamamoto, T. et al. (1993) Biochem. Biophys. Res. Commun. 2:681.
  2. Zhang, S.M. et al. (2008) Innate Immun. 14:175.
  3. Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
  4. Wang, J. et al. (2019) Cell. 176:334.
  5. Yu, HT. et al. (2009) J. Biol. Chem. 284:13335.
  6. Li, C.Y. et al. (2010) Gut. 59:817.
  7. Liu, Z. and C. Ukomadu. (2008) Biochem. Briophys. Res. Commun. 365:729.

Long Name

Fibrinogen Like 1

Alternate Names

FGL1, Fibrinogen-like-protein 1, FREP1

Entrez Gene IDs

2267 (Human); 234199 (Mouse); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Gene Symbol

FGL1

UniProt

BAB70690.1

Additional Hepassocin/FGL1 Products

Product Documents for Recombinant Human Hepassocin/FGL1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human Hepassocin/FGL1 His-tag Protein, CF

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