Recombinant Human Hepsin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4776-SE
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Hepsin protein
Arg45-Leu417 (Asp161Glu & Arg162Lys), with a C-terminal 10-His tag
Arg45-Leu417 (Asp161Glu & Arg162Lys), with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg45
Predicted Molecular Mass
42 kDa
SDS-PAGE
40-43 kDa, reducing conditions
Activity
Measured by its ability to cleave tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC).
The specific activity is >20,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >20,000 pmol/min/μg, as measured under the described conditions.
Reviewed Applications
Read 3 reviews rated 5 using 4776-SE in the following applications:
Formulation, Preparation and Storage
4776-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Hepsin
Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6). Recombinant human Hepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the recombinant human Hepsin.
References
- Leytus, S.P. et al. (1988) Biochemistry 27:1067.
- Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
- Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
- Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
- Dhanasekaran, S.M. et al. (2001) Nature 412:822.
- Wu, Q. and Parry, G. (2007) Front. Biosci. 12:5052.
Alternate Names
HPN, TMPRSS1
Gene Symbol
HPN
UniProt
Additional Hepsin Products
Product Documents for Recombinant Human Hepsin Protein, CF
Product Specific Notices for Recombinant Human Hepsin Protein, CF
For research use only
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