Recombinant Human Hexokinase 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8178-HK

R&D Systems, part of Bio-Techne
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8178-HK-020
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Key Product Details

Source

E. coli

Accession #

P19367

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Hexokinase 1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Hexokinase 1 protein
Phe11-Ser917, with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

102 kDa

SDS-PAGE

95-115 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate glucose.
The specific activity is >700 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8178-HK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT, Glucose and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Hexokinase 1

Hexokinases phosphorylate hexose to form hexose 6-phosphate, the first step in hexose metabolism (1). Phosphorylation of a hexose adds charge to molecule thereby making it difficult to transport out of a cell. The hexose is therefore retained for intracellular metabolic processes, such as glycolysis or glycogen synthesis. In most organisms, glucose is the most important substrate of hexokinases and glucose-6-phosphate is the most important product. There are four mammalian hexokinases (2). Hexokinase 1, 2 and 3 are referred to as high-affinity hexokinases because their Km for glucose is below 1 mM. Hexokinase 4 is specific for glucose and is also referred to as glucokinase (3). Hexokinase I localizes to the outer membrane of mitochondria and is found in all mammalian tissues. The amino acids involved in mitochondrion membrane localization (4) have been removed in the recombinant enzyme. Hexokinase 1 (HK1) contains two homologous halves that are believed to be evolved from a single ancestral hexokinase by gene duplication and fusion (5). While the regulatory function is associated with the N-terminal half, the catalytic site is associated with the C-terminal half. HK1 is insensitive to product inhibition (6). Mutation in the active site of human hexokinase is associated with hexokinase deficiency and severe nonspherocytic hemolytic anemia (7). The enzymatic activity of recombinant human HK1 is measured using a phosphatase-coupled method (8).

References

  1. Aleshin, A.E. et al. (1998) Structure 6:39-50
  2. Takeda, J. et al. (1993) J. Biol. Chem. 268:15200.
  3. Lange, A.J. et al. (1991) Biochem. J. 277:159–163.
  4. Magnani, M. et al., J. Biol. Chem. 266: 502.
  5. Nishi, S. et al. (1988) Biochem. Biophys. Res. Commun. 157:937.
  6. Magnani, M. et al. (1992) Biochem. J. 285:193.
  7. Van Wijk R. et al. (2003) Blood 101:345.
  8. Wu, Z.L. (2011) PLoS One 6:e23172.

Alternate Names

HK1, HKI

Entrez Gene IDs

3098 (Human)

Gene Symbol

HK1

UniProt

P19367

Additional Hexokinase 1 Products

Product Documents for Recombinant Human Hexokinase 1 Protein, CF

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Product Specific Notices for Recombinant Human Hexokinase 1 Protein, CF

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