Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF
R&D Systems, part of Bio-Techne | Catalog # E-304
Key Product Details
Product Specifications
Source
Contains an N-terminal Met-Ser-Tyr-Tyr and 6-His tag
Purity
Predicted Molecular Mass
Activity
Reviewed Applications
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Formulation, Preparation and Storage
E-304
Formulation | Supplied as a solution in HEPES, NaCl and TCEP. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Ubiquitin-activating Enzyme/UBE1
Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa. It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11). UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.
References
- Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
- Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
- Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Schulman, B.A. & J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
- Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
- Haas, A.L. & I.A. Rose (1982) J. Biol. Chem. 257:10329.
- Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
- Nouspikel, T. & P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
- Moundry, P. et al. (2012) Cell Cycle 11:1573.
- Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.
Alternate Names
Gene Symbol
UniProt
Additional Ubiquitin-activating Enzyme/UBE1 Products
Product Documents for Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF
Product Specific Notices for Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF
For research use only