Recombinant Human His6-USP30 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E-582
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP30 protein
Thr57 - Glu517 with a N-terminal Met and a C-terminal 6-His tag
Thr57 - Glu517 with a N-terminal Met and a C-terminal 6-His tag
Purity
>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
54 kDa
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial USP30 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555), or 200-500 nM when digesting recombinant Polyubiquitin chain substrates.
Formulation, Preparation and Storage
E-582
| Formulation | Supplied as a solution in HEPES, NaCl, Glycerol and DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: USP30
Ubiquitin carboxyl-terminal hydrolase 30 (USP30) is a member of the C19 family of peptidases and is tethered to the outer mitochondrial surface via a trans-membrane domain. USP30 activity is antagonistic to the pro-mitophagy signal generated by the activities of E3 Ubiquitin Ligase Parkin (PARK2) and kinase PINK1, which are stimulated by mitochondrial damage. In vivo, USP30 cleaves Ubiquitin from mitochondrial surface proteins such as MIRO1, TOMM20, MFN1, and MFN2. The enzyme displays a strong preference for K6- and K11-linked Polyubiquitin chains in vitro, but has substantially lower activity on Polyubiquitin chains that have been phosphorylated with PINK1.
References
- Bingol B. et al. (2014) Nature 510: 370.
- Cunningham C.N. et al. (2015) Nat Cell Biol. 17: 160.
- Thobois S. (2015) Mov. Disord. 30: 340.
- Wang, Y. et al. (2015) Autophagy 11: 595.
- Wauer T., et al. (2015) EMBO J. 34: 307.
- Yue, W. et al. (2014) Cell Res. 24: 482.
Long Name
Ubiquitin Specific Protease 30
Alternate Names
Deubiquitinating Enzyme 30, Ubiquitin Thioesterase 30
Gene Symbol
USP30
UniProt
Additional USP30 Products
Product Documents for Recombinant Human His6-USP30 Protein, CF
Product Specific Notices for Recombinant Human His6-USP30 Protein, CF
For research use only
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