Recombinant Human His6UBE2S Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E2-690
Will be discontinued when existing inventory is gone.
Key Product Details
Product Specifications
Source
E. coli-derived human UBE2S protein
Met1 - Leu222 with a N-terminal 6-His tag
Met1 - Leu222 with a N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
26 kDa
Activity
Recombinant Human His6-UBE2S is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2S concentration of 0.1-1 μM.
Formulation, Preparation and Storage
E2-690
| Formulation | Supplied as a solution in HEPES, NaCl, TCEP and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: UBE2S
Ubiquitin-conjugating Enzyme E2S (UBE2S), also known as E2-EPF, is a widely expressed protein with a predicted molecular weight of 26 kDa (1). Human UBE2S shares 92% and 93% amino acid sequence identity with mouse and rat UBE2S, respectively. UBE2S expression oscillates in a cell cycle-dependent fashion, and it influences cell cycle progression by mediating the Ubiquitin-dependent proteolysis of mitotic regulators (2-4). UBE2S interacts with APC/C, a Ubiquitin ligase (E3), to catalyze the elongation of Lys11-linked Ubiquitin chains on APC/C substrates that have been mono-ubiquitinated by UbcH10/UBE2C or UbcH5/UBE2D (3-7). UBE2S is overexpressed in multiple human cancers and targets the tumor suppressor pVHL for proteasomal degradation, which stabilizes HIF-1 alpha and promotes tumor progression (8).
References
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Liu, Z. et al. (1992) J. Biol. Chem. 267:15829.
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Tedesco, D. et al. (2007) Neoplasia 9:601.
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Williamson, A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:18213.
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Dimova, N.V. et al. (2012) Nat. Cell Biol. 14:168.
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Garnett, M.J. et al. (2009) Nat. Cell Biol. 11:1363.
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Wu, T. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1355.
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Bremm, A. & D. Komander (2012) Methods Mol. Biol. 832:219.
- Jung, C.R. et al. (2006) Nat. Med. 12:809.
Long Name
Ubiquitin-conjugating Enzyme E2S
Alternate Names
E2-24K, E2-EPF, E2-EPF52, EPF5
Gene Symbol
UBE2S
UniProt
Additional UBE2S Products
Product Documents for Recombinant Human His6UBE2S Protein, CF
Product Specific Notices for Recombinant Human His6UBE2S Protein, CF
For research use only
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