Recombinant Human Histamine N-Methyltransferase/HNMT, CF

R&D Systems, part of Bio-Techne | Catalog # 7637-MT

R&D Systems, part of Bio-Techne
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7637-MT-010
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Key Product Details

Source

E. coli

Accession #

P50135

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Histamine N-Methyltransferase/HNMT Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Histamine N-Methyltransferase/HNMT protein
Ala2-Ala292, with an N-terminal HM and 6-His tag.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His (of HM) and Ser7

Predicted Molecular Mass

34 kDa

SDS-PAGE

32-36 kDa, reducing conditions

Activity

Measured by its ability to methylate histamine.
The specific activity is >425 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7637-MT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Histamine N-Methyltransferase/HNMT

Histamine N-Methyltransferase (HNMT) catalyzes the transfer of the methyl group of S-adenosyl-L-methionine to the Ne2 atom of the imidazole ring in histamine producing methylhistamine (1). Because histamine is a potent mediator of numerous biological events, including the development of allergic inflammation, termination of its action by N-methylation is important event. Two major pathways exist for the inactivation of histamine in mammals (2). The pathway of histamine inactivation through HNMT plays a major role in bronchial epithelial and endothelial cells of the human airways and gut and HNMT is the only enzyme responsible for termination of the neurotransmitter action of histamine in the brain. The human HNMT gene contains a common single nucleotide polymorphism (SNP) that codes for either threonine or isoleucine at residue 105 (3). The 105I variant has decreased activity and lower protein levels relative to 105T. This SNP does not pose a serious risk for histamine accumulation (4).

References

  1. Francis, D. M. (1980) Biochem. J. 187:819.
  2. Maints, L. and Novak, N. (2007) Am. J. Clin. Nutr. 85:1185.
  3. Rutherford. K. et al. (2008) Biochemistry. 47:893.
  4. Garcia-Martin, E. et al. (2009) Pharmacogenomics 10:867.

Long Name

Histamine N-Methyltransferase

Alternate Names

Histamine NMethyltransferase, HMT, HNMT, HNMT-S1, HNMT-S2

Entrez Gene IDs

3176 (Human); 140483 (Mouse); 81676 (Rat)

Gene Symbol

HNMT

UniProt

P50135 (Human)

Additional Histamine N-Methyltransferase/HNMT Products

Product Documents for Recombinant Human Histamine N-Methyltransferase/HNMT, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Histamine N-Methyltransferase/HNMT, CF

For research use only

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