Recombinant Human HMG-CoA Reductase/HMGCR Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9264-HM

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9264-HM-020
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

P04035

Conjugate

Unconjugated

Applications

Enzyme Activity

View all HMG-CoA Reductase/HMGCR Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human HMG-CoA Reductase/HMGCR protein
Ser426-Ala888, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

51 kDa

SDS-PAGE

51-61 kDa, reducing conditions

Activity

Measured by its ability to reduce HMG-CoA with NADPH.
The specific activity is >6,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9264-HM
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: HMG-CoA Reductase/HMGCR

HMG-CoA reductase (HMGCR) is a transmembrane glycoprotein of the endoplasmic reticulum. It is the rate-limiting enzyme in cholesterol biosynthesis and converts HMG-CoA to mevalonate (1). Mevalonate can be converted to cholesterol through a series of enzymatic reactions, and can also serve as the precursor for several nonsterol isoprenoid compounds such as ubiquinone, dolichol, and the isopentenyl group of tRNA (2). The activity of HMGCR is finely regulated by a negative feedback mechanism in which cholesterol and the other end products of the metabolic pathway suppress the enzyme in a multivalent fashion. Cholesterol suppresses the reductase activity primarily by inhibiting the rate of transcription of the reductase gene (3). Cytosolic cholesterol is derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis (4). The well-known inhibitors for HMGCR are statins, a class of hypolipidemic agents used as pharmaceuticals to lower cholesterol levels in individuals at risk from cardiovascular disease due to hypercholesterolemia (5). The recombinant human HMGCR contains only the catalytic domain of the enzyme (6).

References

  1. Goldstein, J. L. and Brown, M. S. (1990) Nature 343:425.
  2. Buhaescu, I. and Izzedine, H. (2007) Clin. Biochem. 40:575.
  3. Reynolds, G. A. et al. (1984) Cell 38:275.
  4. Brown, M. S. and Goldstein, J. L. (1980) J. Lipid Res. 21:505.
  5. Sweetman, S. C. (2009) Martindale: the complete drug reference (36th ed.) pp.1155–434.
  6. Istvan, E.S. et al. (2000) EMBO J. 19:819.

Long Name

3-Hydroxy-3-Methylglutaryl-CoA Reductase

Alternate Names

HMGCR, Hydroxymethylglutaryl-CoA Reductase, LDLCQ3

Entrez Gene IDs

3156 (Human); 15357 (Mouse); 25675 (Rat)

Gene Symbol

HMGCR

UniProt

P04035

Additional HMG-CoA Reductase/HMGCR Products

Product Documents for Recombinant Human HMG-CoA Reductase/HMGCR Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human HMG-CoA Reductase/HMGCR Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.