Recombinant Human HMGN1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8187-HM

R&D Systems, part of Bio-Techne
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8187-HM-050
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Key Product Details

Source

E. coli

Accession #

P05114

Conjugate

Unconjugated

Applications

Bioactivity

View all HMGN1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human HMGN1 protein
Pro2-Asp100

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro2

Predicted Molecular Mass

11 kDa

SDS-PAGE

20 kDa, reducing conditions

Activity

Measured by its ability to induce IL-6 secretion by monocyte-derived dendritic cells.
The ED50 for this effect is 0.6-3 μg/mL

Formulation, Preparation and Storage

8187-HM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HMGN1

High-mobility group nucleosome-binding protein 1 (HMGN1), also known as HMG-14, is a member of the family of non-histone chromosomal proteins that play important roles in chromatin remodeling (1, 2). HMGN1 contains an N-terminal domain that interacts with nucleosomes and a C-terminal domain that assists chromatin unfolding (2, 3). Human HMGN1 shares 80% amino acid sequence identity with mouse and rat HMGN1. Alternative RNA processing generates a long isoform with a 45 amino acid insertion following Met1 (4). The trafficking of HMGN1 between the nucleus and cytoplasm is regulated by its serine phosphorylation at multiple sites (5, 6). In the nucleus, HMGN1 preferentially associates with sites of chromatin remodeling and gene transcription, allowing it to modulate the expression of a wide variety of genes (7, 8). It contributes to DNA damage repair by directly associating with and activating the double strand break sensor PARP-1 (9). HMGN1 is additionally one of several alarmins, proteins that are normally intracellular but are released following tissue damage or necrosis (1). In this setting, HMGN1 promotes dendritic cell maturation, recruitment to sites of inflammation, and production of Th1 inflammatory cytokines (10). These pro-inflammatory activities are dependent on the direct association of HMGN1 with the TLR4-MD2 complex on dendritic cells (10, 11).

References

  1. Yanai, H. et al. (2012) Trends Immunol. 33:633.
  2. Gerlitz, G. (2010) Biochim. Biophys. Acta 1799:80.
  3. Landsman, D. et al. (1986) J. Biol. Chem. 261:16082.
  4. Zougman, A. et al. (2008) Curr. Biol. 18:1760.
  5. Louie, D.F. et al. (2000) Protein Sci. 9:170.
  6. Pogna, E.A. et al. (2010) Biochim. Biophys. Acta 1799:93.
  7. Cuddapah, S. et al. (2011) Mol. Cell. Biol. 31:700.
  8. Kugler, J.E. et al. (2013) J. Biol. Chem. 288:16690.
  9. Masaoka, A. et al. (2012) J. Biol. Chem. 287:27648.
  10. Yang, D. et al. (2010) Biochim. Biophys. Acta 1799:157.
  11. Yang, D. et al. (2012) J. Exp. Med. 209:157.

Long Name

High Mobility Group Nucleosome Binding Domain 1

Alternate Names

HMG14

Entrez Gene IDs

3150 (Human); 15312 (Mouse); 360704 (Rat)

Gene Symbol

HMGN1

UniProt

P05114

Additional HMGN1 Products

Product Documents for Recombinant Human HMGN1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human HMGN1 Protein, CF

For research use only

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