Recombinant Human HS3ST3B1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7276-ST

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
7276-ST-020
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

Q9Y662

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 protein
Ser54-Asp390 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly60

Predicted Molecular Mass

38 kDa

SDS-PAGE

39-52 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to heparan sulfate.
The specific activity is >25 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7276-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3

Heparan sulfate is a highly sulfated polysaccharide found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, is considered to be a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2‑O position by heparan sulfate 2‑O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3‑O, and 6‑O positions by N‑deacetylase/ N‑sulfotransferases (NDSTs), heparan sulfate 3‑O sulfotransferases (HS3STs) and heparan sulfate 6‑O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST3 has two forms, HS3ST3A1 and HS3ST3B1, differing only at the N‑terminus. The two HS3STs have the same substrate specificity (5) and similar tissue distribution with a high levels of expression in the liver and spleen (3, 6). HS3ST3 can sulfate IdoUA2S‑GlcNS, IdoUA2S‑GlcNH2 and IdoUA2S‑GlcNS6S and generate tetrasulfated disaccharide units (3, 6). HS3ST3 is involved in generation of a binding receptor to the herpes simplex virus‑1 (HSV‑1) (7). The enzyme activity was determined using a phosphatase-coupled method (8).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
  4. Xu, D. et al. (2005) Biochem. J. 386:451.
  5. Liu, J. et al. (1999) J. Biol. Chem. 274:5185.
  6. Mochiziki, H. (2008) J. Biol. Chem. 283:31237.
  7. Moon, A.F. et al. (2004) J. Biol. Chem. 279:45185.
  8. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Long Name

Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3B1

Alternate Names

3OST3B1, Heparan Sulfate Glucosamine 3OSulfotransferase 3

Entrez Gene IDs

9953 (Human); 54710 (Mouse); 303218 (Rat)

Gene Symbol

HS3ST3B1

UniProt

Q9Y662 (Human)

Additional Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 Products

Product Documents for Recombinant Human HS3ST3B1 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human HS3ST3B1 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.