Recombinant Human ICAM-2/CD102 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 803-I2
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ICAM-2/CD102 protein
| Human ICAM-2 (Lys25 - Gln223) Accession # CAA33630 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys25
Predicted Molecular Mass
50 kDa (monomer)
SDS-PAGE
86 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When 5 x 104 cells/well are added to rhICAM-2/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >70% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
When 5 x 104 cells/well are added to rhICAM-2/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >70% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
803-I2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol. |
| Reconstitution |
Reconstitute at 0.4 mg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ICAM-2/CD102
Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration.
References
- Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 2
Alternate Names
CD102, ICAM2
Gene Symbol
ICAM2
UniProt
Additional ICAM-2/CD102 Products
Product Documents for Recombinant Human ICAM-2/CD102 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human ICAM-2/CD102 Fc Chimera Protein, CF
For research use only
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