Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 715-IC
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ICAM-3/CD50 protein
| Human ICAM-3 (Gln30 - His485) Accession # CAA49473 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln30 predicted
Predicted Molecular Mass
76.7 kDa (monomer)
SDS-PAGE
120 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When 5 x 104 cells/well are added to rhICAM-3/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), approximately 40-70% will adhere after PMA stimulation for 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
When 5 x 104 cells/well are added to rhICAM-3/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), approximately 40-70% will adhere after PMA stimulation for 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
715-IC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 1 mg/mL in sterile, deionized water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ICAM-3/CD50
Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alphad/ beta2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.
References
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Entrez Gene IDs
3385 (Human)
Gene Symbol
ICAM3
UniProt
Additional ICAM-3/CD50 Products
Product Documents for Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human ICAM-3/CD50 Fc Chimera Protein, CF
For research use only
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