Recombinant Human ICAM-5 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1950-M5
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ICAM-5 protein
| Human ICAM-5 (Ala28-Glu570) Accession # Q9UMF0 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala28
Predicted Molecular Mass
85.1 kDa (monomer)
SDS-PAGE
125-130 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When 5 x 104 cells/well are added to Recombinant Human ICAM-5/Fc Chimera coated plates (25 µg/mL, 100 µL/well), >25% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
When 5 x 104 cells/well are added to Recombinant Human ICAM-5/Fc Chimera coated plates (25 µg/mL, 100 µL/well), >25% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
1950-M5
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 400 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ICAM-5
Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38 - 55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity.
The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4 - 5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).
References
- Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
- Yoshihara, Y. et al. (1994) Neuron 12:541.
- Tain, L. et al. (1997) J. Immunol. 158:928.
- Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
- Tain, L. et al. (2000) J. Immunol. 150:243.
Long Name
Intercellular Adhesion Molecule 5
Alternate Names
ICAM5, Telencephalin
Gene Symbol
ICAM5
UniProt
Additional ICAM-5 Products
Product Documents for Recombinant Human ICAM-5 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human ICAM-5 Fc Chimera Protein, CF
For research use only
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