Recombinant Human IFN-alpha 21/IFNA21 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11159-IF

R&D Systems, part of Bio-Techne
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11159-IF-010
11159-IF-050
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Key Product Details

Source

CHO

Accession #

P01568.2

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-alpha 21/IFNA21 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IFN-alpha 21/IFNA21 protein
Cys24-Glu189

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-22 kDa, under reducing conditions.

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 1.00-30.0 pg/mL.

Scientific Data Images for Recombinant Human IFN-alpha 21/IFNA21 Protein, CF

Recombinant Human IFN-alpha 21/IFNA21 Protein Bioactivity.

Recombinant Human IFN-alpha 21/IFNA21 Protein (Catalog # 11159-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 1.00-30.0 pg/mL.

Recombinant Human IFN-alpha 21/IFNA21 Protein SDS-PAGE.

2 μg/lane of Recombinant Human IFN-alpha 21/IFNA21 Protein (Catalog # 11159-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa, under reducing conditions.

Formulation, Preparation and Storage

11159-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 21/IFNA21

Interferons (IFN) are a family of cytokines with potent antiviral, anti-proliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2-4). Structurally, type I IFNs belong to the class of five helical-bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds (5). There is not a mouse homolog for IFNA21, but mature human IFNA21 is identical to chimpanzee IFNA21. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (6, 7). Individual IFNA subtypes are known to display unique efficacies to viral protection, with IFNA21 displaying intermediate activity inducing interferon stimulating genes (8). Further, human IFNA21 has shown weak anti-viral activity against viruses such as metapneumovirus (9).

References

  1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
  2. Goeddel, D.V. et al. (1980) Nature 287:411.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  5. Wittling, M.C. et al. (2021) Front Immunol. 11:605673.
  6. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  7. James, C.M. et al. (2007) Vaccine. 25(10):1856.
  8. Moll, H.P. et al. (2011) Cytokine. 53:52.
  9. George, J. and Mattapallil, J.J. (2018) Frontiers in immunology 9:299.

Long Name

Interferon alpha F

Alternate Names

IFN-alpha F, IFNA21, IFNalpha F

Entrez Gene IDs

3452 (Human)

Gene Symbol

IFNA21

UniProt

P01568.2

Additional IFN-alpha 21/IFNA21 Products

Product Documents for Recombinant Human IFN-alpha 21/IFNA21 Protein, CF

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Product Specific Notices for Recombinant Human IFN-alpha 21/IFNA21 Protein, CF

For research use only

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