Recombinant Human IGFLR1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8296-IR

R&D Systems, part of Bio-Techne
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8296-IR-050
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Key Product Details

Source

CHO

Accession #

Q9H665

Conjugate

Unconjugated

Applications

Bioactivity

View all IGFLR1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IGFLR1 protein
Ser23-Pro163 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser23

Predicted Molecular Mass

16 kDa

SDS-PAGE

22-32 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IGFLR1 is coated at 0.1 μg/mL (100 μL/well), the concentration of recombinant human IGFL1 that produces 50% optimal binding response is 0.6-3 ng/mL.

Formulation, Preparation and Storage

8296-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFLR1

Insulin Growth Factor-like Family Receptor 1 (IGFLR1) is a 355 amino acid (aa) type 1a transmembrane protein that was identified in a screen for binding partners of human IGFL1 (1). Mature human IGFLR1 consists of an extracellular domain (ECD) with two putative cysteine-rich domains (CRDs), a transmembrane region, and a cytoplasmic domain (1). Its structure has similarities to TNF receptor family members (1).  The ECD contains 12 cysteine residues that are conserved among humans, macaque, mice, and rats (1). Over the first 163 aa, human IGFLR1 shares 61% and 59% aa sequence identity with mouse and rat IGFLR1, respectively. In mice, IGFLR1 is expressed primarily on T cells and, similar to the human proteins, mouse IGFLR1 binds the mouse IGFL protein (1). Human IGFL1 expression is enhanced by TNF-alpha treatment and was shown to be up-regulated in human psoriatic skin samples, suggesting that IGFLR1 may have a role during skin inflammation (1).

References

  1. Lobito, A.A. et al. (2011) J. Biol. Chem. 286:18969.

Long Name

IGF-like Family Receptor 1

Alternate Names

TMEM149, U2AF1L4

Entrez Gene IDs

79713 (Human); 101883 (Mouse); 499126 (Rat)

Gene Symbol

IGFLR1

UniProt

Q9H665

Additional IGFLR1 Products

Product Documents for Recombinant Human IGFLR1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IGFLR1 Protein, CF

For research use only

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