Recombinant Human IGSF21 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10330-S2

R&D Systems, part of Bio-Techne
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10330-S2-050
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Key Product Details

Source

CHO

Accession #

Q96ID5.1

Conjugate

Unconjugated

Applications

Bioactivity

View all IGSF21 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IGSF21 protein
Tyr25-Thr455, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr25

Predicted Molecular Mass

49 kDa

SDS-PAGE

61-68 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IGSF21 His-tag (Catalog # 10330-S2) is immobilized at 1 µg/mL (100 µL/well), it binds to Recombinant Human Neurexin 2 alpha Fc Chimera (Catalog # 6636-NX) with an ED50 of 20-120 ng/mL.

Scientific Data Images for Recombinant Human IGSF21 His-tag Protein, CF

Recombinant Human IGSF21 His-tag Protein Binding Activity

Recombinant Human IGSF21 His-tag Protein Binding Activity

When Recombinant IGSF21 His-tag (Catalog # 10330-S2) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Neurexin 2 alpha Isoform 2 Fc Chimera (Catalog # 6636-NX) binds with an ED50 of 20-120 ng/mL.
Recombinant Human IGSF21 His-tag Protein SDS-PAGE

Recombinant Human IGSF21 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human IGSF21 His-tag (Catalog # 10330-S2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-68 kDa.

Formulation, Preparation and Storage

10330-S2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF21

IGSF21 (Immunoglobulin superfamily member 21) is an approximately 60 kDa glycoprotein that is a member of the immunoglobulin superfamily. In mouse, two isoforms have been identified. The long isoform is predominantly expressed in the brain, while the short isoform ~35 kDa is expressed in other organs (1). IGSF21 is expressed at both embryonic and postnatal stages (1). Human IGSF21 is synthesized as a 467 amino acid (aa) protein that includes a 24 aa signal peptide. The mature protein, which contains two Ig-like domains, shares 95% aa sequence identity with mouse and rat IGSF21. IGSF21 selectively promotes inhibitory presynaptic differentiation through interaction with presynaptic neurexin2 alpha that may represent an important mechanism of inhibitory synapse development in the brain (1). Thalamic IGSF21 expression is altered in response to FGF8 signaling (2). Genotypes of IGSF21 are also reported to influence the development of diabetic retinopathy (3).

References

  1. Tanabe, Y. et al. (2017) Nat. Commun. 8:408.
  2. Botella-Lopez, A. et al. (2019) Brain Struct. Funct. 224:661.
  3. Lin, X. et al. (2016) J. Gene Med. 18:282.

Long Name

Immunoglobulin Superfamily, Member 21

Alternate Names

FLJ41177, IgSF21, immunoglobin superfamily, member 21, immunoglobulin superfamily member 21, MGC15730, RP11-121A23.1

Entrez Gene IDs

84966 (Human); 230868 (Mouse); 298591 (Rat)

Gene Symbol

IGSF21

UniProt

Q96ID5.1

Additional IGSF21 Products

Product Documents for Recombinant Human IGSF21 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IGSF21 His-tag Protein, CF

For research use only

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