Recombinant Human IGSF9B Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9248-S9

R&D Systems, part of Bio-Techne
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9248-S9-050
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Key Product Details

Source

HEK293

Accession #

Q9UPX0

Conjugate

Unconjugated

Applications

Bioactivity

View all IGSF9B Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IGSF9B protein
Glu21-Pro722, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

79 kDa

SDS-PAGE

83-107 kDa, reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 150 ng on a nitrocellulose-coated microplate.

Scientific Data Images for Recombinant Human IGSF9B Protein, CF

Recombinant Human IGSF9B Protein Bioactivity

Recombinant Human IGSF9B Protein Bioactivity

Recombinant Human IGSF9B Inhibits Neurite Outgrowth in E13 Chick Embryonic Dorsal Root Ganglia (DRG) Neurons. A) PBS control coated nitrocellulose microplates showing E13 chick embryonic DRG neurite outgrowth. B) A 3 µl droplet, containing 150 ng of Recombinant Human IGSF9B (Catalog # 9248-S9) coated on nitrocellulose microplates, results in a significant reduction in E13 chick embryonic DRG neurite outgrowth.

Formulation, Preparation and Storage

9248-S9
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF9B

IGSF9B, is an approximately 190 kDa transmembrane glycoprotein that plays a role in neuronal synapse maintenance and function (1). Mature human IGSF9B consists of a 702 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 606 aa cytoplasmic domain. Within the ECD, human IGSF9B shares 97% aa sequence identity with mouse and rat IGSF9B. IGSF9B is widely expressed on interneurons in the brain where it localizes to inhibitory synapses in a ternary complex with Neuroligin-2 and S-SCAM (2). IGSF9B functions as a homophilic adhesion molecule and is required for the maturation of inhibitory synapses on interneurons (2). It shares 37% aa sequence identity with human IGSF9/Dasm1 which is expressed in the brain and dorsal root and trigeminal ganglia (3-6). Dasm1 also functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (6, 7).

References

  1. Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
  2. Woo, J. et al. (2013) J. Cell Biol. 201:929.
  3. Doudney, K. et al. (2002) Genomics 79:663.
  4. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
  5. Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
  6. Mishra, A. et al. (2014) J. Neurosci. 34:4187.
  7. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.

Long Name

Immunoglobulin Superfamily, Member 9B

Alternate Names

immunoglobulin superfamily, member 9B

Entrez Gene IDs

22997 (Human); 235086 (Mouse); 315510 (Rat)

Gene Symbol

IGSF9B

UniProt

Q9UPX0

Additional IGSF9B Products

Product Documents for Recombinant Human IGSF9B Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human IGSF9B Protein, CF

For research use only

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