Recombinant Human IL-12 p35 (C96S) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11209-IL

R&D Systems, part of Bio-Techne
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11209-IL-050
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Key Product Details

Source

HEK293

Accession #

P29459.2

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-12 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-12 protein
Arg23-Ser219 (Cys96Ser)

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg23 

Predicted Molecular Mass

23 kDa 

SDS-PAGE

28-37 kDa, under reducing conditions.

Activity

Measured by its ability to induce IFN-gamma secretion in NK-92 human natural killer lymphoma cells, the concentration of Recombinant Human IL‑12p35 (C96S) Monomer (Catalog # 11209-IL) that produces 50% of the response is 0.300-3.00 ng/mL when Recombinant Human IL‑12p40 Monomer (Catalog # 309-IL) is present at 0.25 µg/mL.

Scientific Data Images for Recombinant Human IL-12 p35 (C96S) Protein, CF

Recombinant Human IL‑12 p35 (C96S) Protein Bioactivity.

Measured by its ability to induce IFN-gamma secretion in NK-92 human natural killer lymphoma cells, the concentration of Recombinant Human IL‑12 p35 (C96S) Monomer (Catalog # 11209-IL) that produces 50% of the response is 0.300-3.00 ng/mL when Recombinant Human IL‑12 p40 Monomer (309-IL) is present at 0.25 µg/mL.

Recombinant Human IL‑12 p35 (C96S) Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL‑12 p35 (C96S) Protein (Catalog # 11209-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 28-37 kDa.

Formulation, Preparation and Storage

11209-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines (1). The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF (2, 3). These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. In addition to be a subunit of the proinflammatory cytokine IL-12, p35 is also a subunit of IL-35, an immune suppressor (4). p35 subunit is broadly expressed while p40 subunit is limited to hematopoietic cells (5). Secretion of p35 is dependent on p40 to ensure correct folding (6). Recent studies show that p40 and p35 which are expressed from two different cell lines can assemble to form functional IL-12 in vitro (7). When functions alone, p35 subunit induces expansion of IL-10 and IL-35 expressing regulatory B cells and inhibits neuroinflammation and ameliorates autoimmune encephalomyelitis (8-9).

References

  1. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.
  2. Gearing, D. et al. (1991) Cell 66:9.
  3. Merberg, D. et al. (1992) Immunology Today 13:78.
  4. Devergne, O. et al. (1997) Proc Natl Acad Sci U.S.A. 94:1204.
  5. Carra, G. et al. (2000) J. Immunol 164:4752.
  6. Reitberger, S. et al. (2017) J. Biol. Chem 292:8073.
  7. Gerber, A.N. et al. (2021) Cell Rep. 37:109816.
  8. Dambuza, I.M. et al. (2017) Nat Commun. 8:719.
  9. Choi, J.K. et al. (2017) Front Immunol. 8:1258.

Long Name

Interleukin 12

Alternate Names

CLMF1, IL12, IL12A, NKSF1

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Gene Symbol

IL12A

UniProt

P29459.2

Additional IL-12 Products

Product Documents for Recombinant Human IL-12 p35 (C96S) Protein, CF

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