Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10930

Biotinylated
R&D Systems, part of Bio-Techne
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AVI10930-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q99665.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-12 R beta 2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-12 R beta 2 protein
Human IL-12 RB2
(Cys28-Asn622)
Accession # Q99665.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys28

Predicted Molecular Mass

96 kDa

SDS-PAGE

115-130 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-12 Protein (Catalog # 219-IL) is immobilized at 1.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL‑12 R beta2 Fc Chimera Avi-tag (Catalog#  AVI10930) that produces 50% of the optimal binding response is 0.15-1.50 μg/mL.

Scientific Data Images for Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF

When Recombinant Human IL-12 Protein (Catalog# 219-IL) is immobilized at 1.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL‑12R beta2 Fc Chimera Avi-tag (Catalog # AVI10930) that produces 50% of the optimal binding response is 0.15-1.50 μg/mL.

Biotinylated Recombinant Human IL‑12 R beta2 Fc Chimera Avi-tag Protein SDS-PAGE.

1 μg/lane of Biotinylated Recombinant Human IL‑12 R beta2 Fc Chimera Avi-tag Protein (Catalog # AVI10930) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized bysilver staining, showing bands at 115-130 kDa and 230-260 kDa, respectively.

Formulation, Preparation and Storage

AVI10930
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 2

IL-12 Receptor beta 2 (IL-12 R beta2) is a member of the cytokine receptor superfamily and when combined with the highly homologous IL-12 R beta1, forms the heterodimeric high affinity IL-12 receptor complex. Both receptors are type I transmembrane glycoproteins with homology to gp130, the common receptor beta-chain of the IL-6-like cytokine superfamily (ref). Mature human IL-12 R beta2 consists of an extracellular domain (ECD) with five fibronectin type III (FnIII) domains and a WSXWS motif, a single transmembrane domain and a cytoplasmic region with a Box 1 motif and a tyrosine phosphorylation site that both mediate intracellular signaling (1). The ECD of human IL-12 R beta2 shares 69% and 67% amino acid sequence identity with mouse and rat IL-12 R beta2, respectively. Human and mouse IL-12 R beta2 do not bind cross-species IL-12 (2). Alternative splicing creates a IL-12 R beta2 with a shortened and altered cytoplasmic sequence (3). IL-12 R beta1 and IL-12 R beta2 are receptors for the IL-12 family, which includes IL-12, IL-23, IL-27 and IL-35 and they activate the Janus kinase (JAK)–STAT (signal transducer and activator of transcription) pathway of signal transduction (4). The receptor chains are utilized by the cytokines in different combinations, with IL-12RB2 binding to IL-12 and IL-35 (5). Unlike IL-12RB1, which is constitutive in T cells, NK cells and B cells, IL-12 R beta2 expression is more limited (2). IL-12 R beta2 is expressed following STAT1 activation by IFN-gamma, IL-27 and/or T cell receptor stimulation of naïve T cells, allowing IL-12 to promote Th1, but not Th2, differentiation (6-8). Among B cells, surface expression is limited to naïve germinal center and memory B cells, and myeloma cells (2). Deletion of mouse IL-12 R beta2 causes systemic overexpression of IL-6, accelerated maturation of thymocytes, deficient regulatory T cell maturation and function, and reduced splenic T cell apoptosis (2, 9-11). These mice are susceptible to autoimmune diseases such as experimental autoimmune encephalitis and spontaneous B cell malignancies (2, 9-11). In humans, polymorphism of the IL-12 R beta2 gene is associated with systemic sclerosis (12). Our Avi-tag Biotinylated IL-12 R beta2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
  2. Pistoia, V. et al. (2009) J. Clin. Oncol. 27:4809.
  3. Poltorak, A. et al. (2001) J Immunol 167:2106.
  4. Vignali, D.A. and Kuchroo, V.K. (2012) Immunol. 13:722.
  5. Jones, L.L. and Vignali, D.A. (2011) Immunol Res. 51:5.
  6. Rogge, L. et al. (1997) J. Exp. Med. 185:825.
  7. Becskei, A. and M.J. Grusby (2007) FEBS Lett. 581:5199.
  8. Szabo, S.J. et al. (1997) J. Exp. Med. 185:817.
  9. Zhao, Z. et al. (2008) J. Immunol. 181:3870.
  10. Gran, B. et al. (2010) Exp. Mol. Pathol. 89:126.
  11. Airoldi, I. et al. (2005) Blood 106:3846.
  12. Bossini-Castillo, L. et al. (2012) Hum. Mol. Genet. 21:926.

Long Name

Interleukin 12 Receptor beta 2

Alternate Names

IL-12Rb2, IL12R beta 2, IL12RB2

Entrez Gene IDs

3595 (Human); 16162 (Mouse)

Gene Symbol

IL12RB2

UniProt

Q99665.1

Additional IL-12 R beta 2 Products

Product Documents for Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF

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