Recombinant Human IL-13 R alpha 2 His-tag, CF

Name: Recombinant Human IL-13 R alpha 2 His-tag, CF
Brand: R&D Systems
SKU: 11562-IR

R&D Systems, part of Bio-Techne | Catalog # 11562-IR

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Key Product Details

Accession #:	Q14627.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-13 R alpha 2 His-tag, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human IL-13 R alpha 2 His-tag, CF

N-terminal sequence Analysis	Pro 43
Predicted Molecular Mass	36 kDa
SDS-PAGE	36 kDa

Scientific Data Examples for Recombinant Human IL-13 R alpha 2 His-tag, CF

	Recombinant Human IL-13 R alpha 2 His-tag Protein Bioactivity. Recombinant Human IL-13 R alpha 2 His-tag Protein (Catalog # 11562-IR) inhibits Recombinant Human IL-13 (213-ILB) induced proliferation in the TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 4.00-60.0 ng/mL.
	Recombinant Human IL-13 R alpha 2 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human IL-13 R alpha 2 His-tag Protein (Catalog # 11562-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-58 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11562-IR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-13 R alpha 2

Interleukin‑13 Receptor alpha 2 (IL‑13 R alpha 2), also known as IL‑13 binding protein, and CD213a2, is a widely expressed 55 kDa cytokine receptor that plays an important role in the Th2‑polarized immune responses characteristic of a variety of pathologies, including parasitic infections and allergic asthma (1, 2). Mature human IL‑13 R alpha 2 consists of a 317 amino acid (aa) extracellular domain with three fibronectin type‑III domains, a WSxWS motif, a 20 aa transmembrane segment, and a 17 aa cytoplasmic domain (3). Within the ECD, human IL‑13 R alpha 2 shares 64% and 62% aa sequence identity with mouse and rat IL‑13 R alpha 2, respectively. In both mouse and human, a 40 kDa‑50 kDa soluble form of IL‑13 R alpha 2 can be generated by MMP‑8 mediated shedding in vitro (4). Although this is assumed to occur in vivo in mouse, there is no evidence that shedding occurs in human (5‑7). In mouse, alternative splicing also leads to sIL‑13 R alpha 2, but again, this phenomenon apparently does not occur in human (6‑7). Thus, the biological effects of human IL‑13 R alpha 2 would appear to be mediated exclusively by membrane IL‑13 R alpha 2 (7). The biological effects of IL‑13 and IL‑4 are closely related in part due to a shared receptor system. IL‑13 binds to IL‑13 R alpha 1 which then forms a signaling complex with IL‑4 R alpha (8, 9). IL‑13 R alpha 2 functions as a decoy receptor by binding and internalizing IL‑13 and preventing it from signaling through the IL‑13 R alpha 1/IL‑4 R alpha complex (3, 10). IL‑13 R alpha 2 can also block IL‑4 induced responses by inhibiting IL‑4 bound IL‑13 R alpha 1/IL‑4 R alpha receptor complexes even though it does not itself bind IL‑4 (11, 12). Aside from its decoy function, IL‑13‑activated IL‑13 R alpha 2 directly promotes the development of tissue fibrosis by inducing the transcription of TGF‑ beta (13). Presumably, any human soluble IL‑13 R alpha 2, if it exists, will retain its ligand binding capability and attenuate responses to IL‑13 but not to IL‑4 (11, 14). The up‑regulation of transmembrane during Th2‑biased immune responses limits the extent of those responses (15‑17).

Item	Value
References	Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338. Caput, D. et al. (1996) J. Biol. Chem. 271:16921. Chen, W. et al. (2008) J. Allergy Clin. Immunol. 122:625. O’Toole, M. et al. (2008) Clin. Exp. Allergy 38:594. Chen, W. et al. (2009) J. Immunol. 183:7870. Kasaian, M.T. et al. (2011) J. Immunol. 187:561. Andrews, A.-L. et al. (2006) J. Immunol. 176:7456. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687. Morimoto, M. et al. (2009) J. Immunol. 183:1934. Zheng, T. et al. (2008) J. Immunol. 180:522.
Long Name	Interleukin 13 Receptor alpha 2
Entrez Gene IDs	3598 (Human); 16165 (Mouse)
Alternate Names	CD213a2, CD213a2 antigen, CT19, IL-13 R alpha 2, IL-13 receptor subunit alpha-2, IL-13R, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13Ra2, IL13BP, IL13R, IL13R alpha 2, IL13RA2, Interleukin-13-binding protein, cancer/testis antigen 19, interleukin 13 binding protein, interleukin 13 receptor alpha 2 chain, interleukin 13 receptor, alpha 2, interleukin-13 receptor subunit alpha-2

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Recombinant Human IL-13 R alpha 2 His-tag, CF

R&D Systems, part of Bio-Techne | Catalog # 11562-IR

Key Product Details

Product Specifications for Recombinant Human IL-13 R alpha 2 His-tag, CF

Scientific Data Examples for Recombinant Human IL-13 R alpha 2 His-tag, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IL-13 R alpha 2

Customer Reviews for Recombinant Human IL-13 R alpha 2 His-tag, CF

Product Documents for Recombinant Human IL-13 R alpha 2 His-tag, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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