Recombinant Human IL-21R Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI991

Biotinylated
R&D Systems, part of Bio-Techne
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AVI991-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-21R Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-21R protein
Human IL-21R
(Cys20-Pro236)
Accession # Q9HBE5.1		 DIEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys20

Predicted Molecular Mass

53 kDa

SDS-PAGE

69-84 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-21 Protein  (Catalog # 8879-IL) is immobilized at 2.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human IL-21R Fc Chimera Avi-tag (Catalog # AVI991) that produces 50% of the optimal binding response is 0.400-2.40 μg/mL.

Scientific Data Images for Recombinant Human IL-21R Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human IL-21R Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human IL-21 Protein (8879-IL) is immobilized at 2.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human IL-21R Fc Chimera Avi-tag Protein (Catalog # AVI991) that produces 50% of the optimal binding response is 0.400 2.40 μg/mL.

Biotinylated Recombinant Human IL-21R Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human IL-21R Fc Chimera Avi-tag Protein (Catalog # AVI991) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 69-84 kDa and 140-170 kDa, respectively.

Formulation, Preparation and Storage

AVI991
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21R

Interleukin-21 Receptor (IL-21 R) is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common  gamma chain ( gammac) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 (2, 3). Mature human IL-21 R consists of a 213 amino acid (aa) extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 253 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, human IL-21 R shares 69% aa identity with mouse and rat IL-21 R, respectively. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8+ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma delta T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15). Our Avi-tag Biotinylated human IL-21R Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
  2. Asao, H. et al. (2001) J. Immunol. 167:1.
  3. Habib, T. et al. (2002) Biochemistry 41:8725.
  4. Parrish-Novak, et al. (2000) Nature 408:57.
  5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  6. Ozaki, K. et al. (2002) Science 298:1630.
  7. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  8. King, I.L. et al. (2010) J. Immunol. 185:6138.
  9. Kasaian, M.T. et al. (2002) Immunity 16:559.
  10. Frohlich, A. et al. (2009 Science 324:1576.
  11. Tortola, L. et al. (2010) Blood 116:5200.
  12. Lee, Y. et al. (2015) J. Clin. Invest. 125:4011.
  13. Moser, E.K. et al. (2015) PLoS One 10:e0120169.
  14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  15. Sarosiek, K.A. et al. (2010) Blood 115:570.

Long Name

Interleukin 21 Receptor

Alternate Names

CD360, IL-21 R, IL21R, NILR

Entrez Gene IDs

50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)

Gene Symbol

IL21R

Additional IL-21R Products

Product Documents for Recombinant Human IL-21R Fc Chimera Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human IL-21R Fc Chimera Avi-tag Protein, CF

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