Recombinant Human IL-32 beta Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6769-IL

R&D Systems, part of Bio-Techne
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6769-IL-025
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Key Product Details

Source

E. coli

Accession #

NP_001012649

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-32 beta Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-32 beta protein
MNHKVHHHHHH Human IL-32 beta
(Met1-Lys188)
Accession # NP_001012649
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

23.1 kDa

Activity

Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells under serum free conditions in the presence of 10 µg/mL of Polymyxin B. Kim, S.H. et al. (2005) Immunity 22:131.
The ED50 for this effect is 10-50 ng/mL.

Reviewed Applications

Read 1 review rated 4 using 6769-IL in the following applications:

Formulation, Preparation and Storage

6769-IL
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and CHAPS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IL-32 beta

Interleukin 32 (IL‑32) is an N‑glycosylated cytokine that is up-regulated by inflammatory stimulation in monocytes, NK cells, epithelial cells, and vascular endothelial cells as well as in activated T cells (1‑6). It cooperates with inflammatory stimuli to promote the expression of other proinflammatory molecules such as TNF‑ alpha, IL‑6, IL‑1 beta, IL‑1 alpha, and CXCL8/IL‑8 (5‑9). The longest of several IL‑32 splicing variants is the 234 amino acid gamma isoform which is also known as natural killer cell transcript 4 (NK4) (9). The 25 kDa beta isoform (IL‑32 beta) lacks aa 19‑64 including a portion of the putative signal peptide. Neutrophil‑derived Proteinase 3 (PR3) cleaves IL‑32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL‑32 isoforms (10). The alpha, beta, gamma, delta, epsilon, and zeta isoforms show different degrees of antiviral activity against influenza virus replication with IL‑32 gamma being the most potent (11). IL‑32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (6, 12‑14). IL‑32 inhibits HIV‑1 replication in vitro, and it is elevated in the serum of HIV‑1 patients (15, 16).

References

  1. Netea, M.G. et al. (2006) PloS Med. 3:e277.
  2. Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. 106:3883.
  3. Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
  4. Goda, C. et al. (2006) Int. Immunol. 18:233.
  5. Choi, J.-D. et al. (2009) Immunology 126:535.
  6. Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
  7. Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. 102:16309.
  8. Hong, J. et al. (2010) Cytokine 49:171.
  9. Kim, S.-H. et al. (2005) Immunity 22:131.
  10. Novick, D. et al. (2006) Proc. Natl. Acad. Sci. 103:3316.
  11. Li, W. et al. (2010) J. Immunol. 185:5056.
  12. Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
  13. Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. 103:3298.
  14. Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
  15. Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
  16. Nold, M.F. et al. (2008) J. Immunol. 181:557.

Long Name

Interleukin 32 beta

Alternate Names

IL32 beta

UniProt

NP_001012649

Additional IL-32 beta Products

Product Documents for Recombinant Human IL-32 beta Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human IL-32 beta Protein, CF

For research use only

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