Recombinant Human IL-37/IL-1F7 Protein

R&D Systems, part of Bio-Techne | Catalog # 9225-IL

R&D Systems, part of Bio-Techne
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Carrier Free
9225-IL-025/CF
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With Carrier
9225-IL-025
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Key Product Details

Source

E. coli

Accession #

Q9NZH6-2

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-37/IL-1F7 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-37/IL-1F7 protein
Ser2-Asp192

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

21 kDa

SDS-PAGE

22 kDa, reducing conditions

Activity

Measured by its ability to inhibit LPS-stimulated IL-8 secretion by HK-2 human kidney epithelial cells.
The ED50 for this effect is 0.25-1.25 μg/mL.

Scientific Data Images for Recombinant Human IL-37/IL-1F7 Protein

Recombinant Human IL-37/IL-1F7 Protein Bioactivity

Recombinant Human IL-37/IL-1F7 Protein Bioactivity

Recombinant Human IL-37/IL-1F7 (Catalog # 9225-IL) inhibits LPS-stimulated IL-8 secretion by HK-2 human kidney epithelial cells. The ED50 for this effect is 0.25-1.25 μg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9225-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9225-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-37/IL-1F7

Human Interleukin-1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family (1-3). Alternative splicing generates multiple isoforms (IL-37a through e) with deletions in the N-terminal region of the molecule (4-7). IL-37b shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta, and IL-1ra, respectively. Mouse IL-37 has not been reported, but human IL-37b is active on mouse cells. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-37 variants lack a typical signal peptide. IL-37b is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (8). Experimental over-expression of IL-37b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (8, 9). Both unprocessed and mature IL-37b can form homodimers in solution (8, 10). Although IL-37b will bind to IL-18 R alpha with low affinity, this has no effect on IL‑18 receptor activity (7, 10, 11). Alternatively, IL-37b will also bind to IL-18 BP, generating a complex that interacts with IL-18 R beta. This has the effect of attenuating IL‑18 activity via the IL-18 receptor (11). IL-37b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-37b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (12).

References

  1. Dinarello, C.A. and P. Bufler (2013) Sem. Immunol. 25:466.
  2. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127.
  3. Ding, V.A. et al. (2016) Med. Oncol. 33:68.
  4. Smith, D.E. et al. (2000) J. Biol Chem 275:1169.
  5. Kumar S. et al. (2000) J. Biol Chem 275:10308.
  6. Busfield S.J. et al. (2000) Genomics 66:213.
  7. Pan, G. et al. (2000) Cytokine 13:1.
  8. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014.
  9. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711.
  10. Kumar S. et al. (2002) Cytokine 18:61.
  11. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
  12. Sharma, S. et al. (2008) J. Immunol. 180:5477.

Long Name

Interleukin 37

Alternate Names

FIL1 zeta, IL-1F7, IL37

Entrez Gene IDs

27178 (Human)

Gene Symbol

IL37

UniProt

Q9NZH6-2

Additional IL-37/IL-1F7 Products

Product Documents for Recombinant Human IL-37/IL-1F7 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 9225-IL (with carrier)
Download SDS 9225-IL/CF (carrier free)

Product Specific Notices for Recombinant Human IL-37/IL-1F7 Protein

For research use only

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