Recombinant Human IL1RAPL1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9949-MR

R&D Systems, part of Bio-Techne
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9949-MR-050
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Key Product Details

Source

CHO

Accession #

Q9NZN1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL1RAPL1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL1RAPL1 protein
Human IL1RAPL1
(Leu19-Thr357)
Accession # Q9NZN1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu19

Predicted Molecular Mass

66 kDa

SDS-PAGE

81-92 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PTPRD Fc Chimera is coated at 1 µg/mL, 100 μL/well, Recombinant Human IL1RAPL1 binds with an ED50 of 5-30 ng/mL.

Scientific Data Images for Recombinant Human IL1RAPL1 Protein, CF

Recombinant Human IL1RAPL1 Protein Binding Activity

Recombinant Human IL1RAPL1 Protein Binding Activity

When Recombinant Human PTPRD Fc Chimera is coated at 1 µg/mL, Recombinant Human IL1RAPL1 Fc Chimera binds with an ED50 of 5-30 ng/mL.
Recombinant Human IL1RAPL1 Protein SDS-PAGE

Recombinant Human IL1RAPL1 Protein SDS-PAGE

2 μg/lane of Recombinant Human IL1RAPL1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 81-92 kDa and 160-180 kDa, respectively.

Formulation, Preparation and Storage

9949-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IL1RAPL1

Interleukin 1 receptor accessory protein-like 1 (IL1RAPL1), also known as Oligophenin-4 (OPHN4) and three immunoglobulin domain containing IL-1 receptor-related 2 (TIGIRR-2) (1), is a member of the IL-1 receptor superfamily. IL1RAPL1 is a single pass type I membrane protein which contains an N-terminal signal peptide (aa 1-18 ), three extracellular immunoglobulin-like domains (aa 19-350), a transmembrane domain (aa 358-378 ), an intracellular Toll/IL-1R domain (aa 403-562), and a long C-terminal tail which interacts with multiple signaling molecules (aa 549-644 ) (2). High expression levels of IL1RAPL1 was found in post-natal hippocampus, and its expression is upregulated by neuronal activity (3). The extracellular domain of IL1RAPL1 can mediate synapse formation through trans-synaptic interaction with PTPRD (4, 5). In neurons, IL1RAPL1 interacts with PSD-95, a major scaffolding protein of excitatory synapses, and modulates its synaptic localization by regulating JNK activity and PSD-95 phosphorylation (3).  Mutation or deletion of IL1RAPL1 gene is associated with non-syndromic intellectual disability and autism spectrum disorder (5). Human IL1RAPL1 shares 98% and 99% aa sequence identity with mouse and rat IL1RAPL1, respectively.

References

  1. Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
  2. Bahi, N. et al. (2003) Hum. Mol. Gen. 12:1415.
  3. Pavlowsky, A. et al. (2010) Curr. Biol. 20:103.
  4. Yoshida, T. et al. (2011) J. Neurosci. 31:13485.
  5. Ramos-Brossier, M. et al. (2015) Hum Mol Genet. 24:1106.

Long Name

Interleukin 1 Receptor Accessory Protein-like 1

Alternate Names

IL-1R9, IL-1RAPL1, TIGIRR-2

Entrez Gene IDs

11141 (Human); 331461 (Mouse); 317553 (Rat)

Gene Symbol

IL1RAPL1

UniProt

Q9NZN1

Additional IL1RAPL1 Products

Product Documents for Recombinant Human IL1RAPL1 Protein, CF

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Product Specific Notices for Recombinant Human IL1RAPL1 Protein, CF

For research use only

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