Recombinant Human INSRR Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9566-RR

R&D Systems, part of Bio-Techne
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9566-RR-050
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Key Product Details

Source

CHO

Accession #

P14616

Structure / Form

Disulfide-linked heterotetramer

Conjugate

Unconjugated

View all INSRR Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human INSRR protein
  
Human INSRR beta
(Ala747-Leu923)
Accession # P14616		 HHHHHH
Human INSRR alpha
(Leu24-Gly742)
Accession # P14616
Human INSRR alpha
(Leu24-Gly742)
Accession # P14616
Human INSRR beta
(Ala747-Leu923)
Accession # P14616		 HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24 (INSRR alpha ) & Ala747 (INSRR beta )

Predicted Molecular Mass

100 kDa

SDS-PAGE

30-39 kDa, 93 - 116 kDa, 124 - 144 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9566-RR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: INSRR

Insulin Receptor-related Receptor (INSRR) is a type I transmembrane glycoprotein in the Insulin/IGF receptor family of receptor tyrosine kinases (1). The mature receptor contains an extracellular alpha subunit and an extracellular/transmembrane/cytoplasmic beta subunit. The amino acid 1-923 encodes the extracellular domain. Alternative splicing generates A and B isoforms which may homodimerize or heterodimerize with the IGF-I receptor (2). INSRR signaling regulates glucose uptake and metabolism but also contributes to cell growth, differentiation and apoptosis. INSRR is specifically and reversibly activated by alkaline media through a positive cooperativity mechanism. It also triggers receptor conformational changes and elicits intracellular signaling. INSRR is predominantly expressed in organs that come in contact with mildly alkaline media, and is postulated to be an alkali sensor to manage metabolic bicarbonate excess in kidney (3). Mutations in INSRR have been linked to severe insulin resistance (type A and Rabson-Mendenhall syndrome), type II diabetes mellitus, and leprechaunism (Donohue syndrome) (4, 5). Human INSRR shares 89% identity with mouse and rat INSRR.

References

  1. Nef, S. et al. (2003) Nature 426:291.
  2. Lee, J. & Pilch, P. F. (1994) Am J Physiol Cell Physiol. 266:C319.
  3. Deyev, I. E. et al. (2011) Cell Metab. 13:679.
  4. Kobayashi, M. et al. (1978). Proc Natl Acad Sci U.S.A. 75:3469.
  5. Musso, C. et al. (2004). Medicine 83:209.

Long Name

Insulin Receptor-related Receptor

Alternate Names

IRR

Entrez Gene IDs

3645 (Human); 23920 (Mouse)

Gene Symbol

INSRR

UniProt

P14616

Additional INSRR Products

Product Documents for Recombinant Human INSRR Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human INSRR Protein, CF

For research use only

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