Recombinant Human Insulin R/CD220 (aa 28-956) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8974-IR

B Isoform
R&D Systems, part of Bio-Techne
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8974-IR-050
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Key Product Details

Source

HEK293

Accession #

P06213.4

Structure / Form

Disulfide-linked heterotetramer

Conjugate

Unconjugated

Applications

Bioactivity

View all Insulin R/CD220 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Insulin R/CD220 protein
His28-Arg762( alpha subunit) and Ser763-Lys956 with a c-terminal 10-His tag ( beta subunit)

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His28 ( alpha subunit), Ser763 ( beta subunit)

Predicted Molecular Mass

107 kDa ( alpha & beta heterodimer)

SDS-PAGE

105-120 kDa ( alpha subunit) and 37-49 kDa ( beta subunit), reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220 B Isoform, the concentration of Recombinant Human Insulin R/CD220 B Isoform that produces 50% of the optimal binding response is 0.1-0.5 μg/mL.

Formulation, Preparation and Storage

8974-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Insulin R/CD220

The Insulin Receptor (gene name INSR, designated CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases that share structural similarity and overlapping intracellular signaling events (1-3). The 1382 amino acid (aa) human Insulin R preproprotein (B isoform) is processed by proteolysis to remove the signal peptide and produce an extracellular alpha portion (aa 28-762), and an extracellular/transmembrane/cytoplasmic beta subunit (aa 763-1382) (4). The extracellular domain (ECD) contains two homologous globular domains separated by a cysteine-rich domain and followed by three fibronectin type III domains. The intracellular region contains insulin-receptor substrate (IRS) docking sites, the kinase domain, and a phosphotyrosine-containing linker region. The human Insulin R ECD shares 96% aa sequence identity with mouse, rat, equine and canine Insulin R. As a result of alternative splicing, two INSR isoforms that differ by the absence (IR-A) or presence (IR-B) of a 12 aa residue sequence in the carboxyl terminus of the alpha subunit exist (4). IR-A expression is highest in fetal tissues and cancer cells, while IR-B is concentrated in adult differentiated cells (2-5). IR-A and IR-B may homodimerize, or heterodimerize with the IGF-I receptor (1, 3, 4). All receptor combinations bind insulin, IGF-I or IGF-II, but with differing affinities; for example, IR-A has considerably higher affinity for IGF-II as compared to IR-B (2-5). This system allows fine tuning of signaling pathways according to the concentrations of insulin, IGF-I and IGF-II, and expression of receptor subunits on the cell surface (2, 3). Insulin R signaling regulates glucose uptake and metabolism, but also contributes to cell growth, differentiation and apoptosis (2, 3, 5, 6). Mutations in the Insulin R gene have been linked severe insulin resistance (type A and Rabson-Mendenhall syndrome) that may include type II diabetes mellitus and, rarely, leprechaunism (Donohue syndrome) that also includes growth delays and endocrine system abnormalities (1, 7). This human Insulin R product consists of the entire ECD of the IR-B isoform.

References

  1. Nakae, J. et al. (2001) Endoc. Rev. 22:818.
  2. Sciacca, L. et al. (2003) Endocrinology 144:2650.
  3. Belfiore, A. et al. (2009) Endocrine Rev. 30:586.
  4. Lawrence, M.C. et al. (2007) Curr. Opin. Struct. Biol. 17:699.
  5. Sacco, A. et al. (2009) Endocrinology 150:3594.
  6. Kitamura, T. et al. (2004) J. Clin. Invest. 113:209.
  7. Musso, C. et. al. (2004) Medicine (Baltimore) 83: 209.    

Long Name

Insulin Receptor

Alternate Names

CD220, INSR, InsulinR

Entrez Gene IDs

3643 (Human); 16337 (Mouse)

Gene Symbol

INSR

UniProt

P06213.4

Additional Insulin R/CD220 Products

Product Documents for Recombinant Human Insulin R/CD220 (aa 28-956) Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Insulin R/CD220 (aa 28-956) Protein, CF

For research use only

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