Recombinant Human Intelectin-1/Omentin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9137-IN

R&D Systems, part of Bio-Techne
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9137-IN-050
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Key Product Details

Source

NS0

Accession #

BAA96094

Structure / Form

Disulfide-linked homotrimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Intelectin-1/Omentin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Intelectin-1/Omentin protein
Trp17-Arg313

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp17 & Thr19

Predicted Molecular Mass

33 kDa

SDS-PAGE

36-42 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Streptococcus Pneumococcal Polysaccharide Type 70 is coated at 2 µg/mL (100 μL/well), Recombinant Human Intelectin-1/Omentin binds with a typical ED50 of 3-15 μg/mL.

Reviewed Applications

Read 1 review rated 4 using 9137-IN in the following applications:

Scientific Data Images for Recombinant Human Intelectin-1/Omentin Protein, CF

Recombinant Human Intelectin-1/Omentin Protein Bioactivity

Recombinant Human Intelectin-1/Omentin Protein Bioactivity

When Streptococcus Pneumococcal Polysaccharide Type 70 is coated at 2 µg/mL (100 μL/well), Recombinant Human Intelectin-1/Omentin binds (Catalog # 9137-IN) with a typical ED50 of 3-15 μg/mL.

Formulation, Preparation and Storage

9137-IN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris.
Reconstitution
Reconstitute at 500 μg/mL in Water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-1/Omentin

Intelectin-1, also known as Omentin, HL-1, and Lactoferrin Receptor, is a multifunctional protein that plays a protective role in the response to cardiovascular injury (1). Mature human Intelectin-1 contains one fibrinogen C-terminal domain (2-4) and shares 82% and 80% aa sequence identity with mouse and rat Intelectin-1, respectively. Intelectin-1 is expressed by vascular smooth muscle and endothelial cells (3, 5, 6), particularly in the heart, small intestine, colon, thymus, ovary, testis, and omental fat (2, 3, 6) as well as by intestinal Paneth cells and airway and intestinal goblet cells (7-9). It is presented as a 35-40 kDa GPI-anchored glycoprotein that forms disulfide-linked homotrimers (2, 4) and can be released into the circulation and bronchiolar lavage fluid (6, 8). It binds to microbial galactofuranosyl carbohydrates (2, 11) and additionally functions as a receptor for the iron-transporting protein Lactoferrin (4). Following cardiovascular injury, Intelectin-1 limits fibrosis, cardiac hypertrophy, and carotid artery intimal hyperplasia (12, 13) foam cell generation [5], smooth muscle cell proliferation, migration, and calcification (5, 12, 14), and it also promotes revascularization (15). In addition, Intelectin-1 inhibits osteoblast bone matrix mineralization (14), enhances
insulin-stimulated uptake by adipocytes (6), supports neural stem cell viability (16), and inhibits the invasion and metastasis of neuroblastoma cells (17).

References

  1. Jaikanth, C. et al. (2013) Exp. Clin. Endocrinol. Diabetes 121:377.
  2. Tsuji, S. et al. (2001) J. Biol. Chem. 276:23456.
  3. Lee, J.-K. et al. (2001) Glycobiology 11:65.
  4. Suzuki, Y.A. et al. (2001) Biochemistry 40:15771.
  5. Watanabe, K. et al. (2016) Cardiovasc. Res. PMID 26790473.
  6. Yang, R.-Z. et al. (2006) Am. J. Physiol. Endocrinol. Metab. 290:E1253.
  7. Komiya, T. et al. (1998) Biochem. Biophys. Res. Commun. 251:759.
  8. Kerr, S.C. et al. (2014) Am. J. Respir. Crit. Care Med. 189:1005.
  9. Washimi, K. et al. (2012) PLoS One 7:e39889.
  10. Tsuji, S. et al. (2007) Glycobiology 17:1045.
  11. Wesener, D.A. et al. (2015) Nat. Struct. Mol. Biol. 22:603.
  12. Matsuo, K. et al. (2015) J. Mol. Cell. Cardiol. 79:195.
  13. Kazama, K. et al. (2014) Am. J. Physiol. Heart Circ. Physiol. 306:H1714.
  14. Xie, H. et al. (2011) Cardiovasc. Res. 92:296.
  15. Maruyama, S. et al. (2012) J. Biol. Chem. 287:408.
  16. Zhao, L.R. et al. (2015) Mol. Med. Rep. 11:1859.
  17. Li, D. et al. (2015) Mol. Cancer 14:47.

Alternate Names

HL-1, Intelectin1, ITLN1, Itlna, LFR, Omentin, Omentin-1

Entrez Gene IDs

55600 (Human); 16429 (Mouse)

Gene Symbol

ITLN1

UniProt

BAA96094

Additional Intelectin-1/Omentin Products

Product Documents for Recombinant Human Intelectin-1/Omentin Protein, CF

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