Recombinant Human ISLR-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4562-R2

R&D Systems, part of Bio-Techne
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4562-R2-050
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Key Product Details

Source

NS0

Accession #

Q6UXK2

Conjugate

Unconjugated

Applications

Bioactivity

View all ISLR-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ISLR-2 protein
Cys20-Leu590, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys20

Predicted Molecular Mass

61 kDa

SDS-PAGE

85-95 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.
The ED50 for this effect is 200-800 ng/mL.

Formulation, Preparation and Storage

4562-R2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ISLR-2

ISLR2 (Immunoglobulin Superfamily containing Leucine-rich Repeat protein 2; also known as LINX/Leucine-rich repeat domain and Immunoglobulin domain containing axoN eXtension protein and Mbu-3 [in mouse]) is a member of the LIG superfamily of molecules (1, 2). Members of this family are typically integral membrane proteins that are characterized by the presence of multiple LRRs (leucine-rich repeats) N-terminal to one Ig domain.  ISLR2 should not be confused with an identically‑named, widely-expressed transcript isoform containing the ISLR1 protein (3). ISLR2 is principally associated with the embryonic nervous system.  In particular, it appears to coexist with TRKs on both sensory and motor postmitotic neurons (2, 4, 5). On these spinal neuron cell types, ISLR2 (plus other LIG family members) is suggested to fine-tune TrkA, TrkC and Ret downstream signaling in a temporally-restricted manner. This is posited to impact axonal outgrowth, branching and fasciculation (5). Notably, a lack of ISLR2 does not abrogate TrkA signaling. This suggests a modulating role for the molecule, and is consistent with the observation that ISLR2 and Trk interact through their extracellular domains (4, 5). Mature human ISLR2 is a 100-120 kDa, 727 amino acid (aa) type I transmembrane protein. It contains a 571 aa extracellular region (aa 19-589) plus a 135 aa cytoplasmic domain (2, 6). The extracellular region possesses seven LRR repeats (aa 19‑232) coupled to one Ig-like domain (aa 233-371).  Human and mouse extracellular domains share 89% aa sequence  identity (6).

References

  1. MacLaren, C.M. et al. (2004) Dev. Genes Evol. 214:360.
  2. Gejima, R. et al. (2006) Gene Expr. Patterns 6:235.
  3. Nagasawa, A. et al. (1999) Genomics 61:37.
  4. Homma, S. et al. (2009) Gene Expr. Patterns 9:1.
  5. Mandai, K. et al. (2009) Neuron 63:614.
  6. Lee, C.J. et al. (2008) BMB Rep. 41:875.

Long Name

Immunoglobulin Superfamily Containing Leucine-rich Repeat 2

Alternate Names

ISLR2

Entrez Gene IDs

57611 (Human); 320563 (Mouse)

Gene Symbol

ISLR2

UniProt

Q6UXK2

Additional ISLR-2 Products

Product Documents for Recombinant Human ISLR-2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human ISLR-2 Protein, CF

For research use only

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