Recombinant Human ITIH4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10269-IT

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

AAI36394.1

Conjugate

Unconjugated

Applications

Bioactivity

View all ITIH4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ITIH4 protein
Glu29-Leu930, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu29

Predicted Molecular Mass

101 kDa

SDS-PAGE

113-117 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ITIH4 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human TSG‑6 (Catalog # 2104-TS) that produces 50% of the optimal binding response is 5-25 μg/mL.

Scientific Data Images for Recombinant Human ITIH4 His-tag Protein, CF

Recombinant Human ITIH4 His-tag Protein SDS-PAGE

Recombinant Human ITIH4 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human ITIH4 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 115 kDa under reducing conditions.

Formulation, Preparation and Storage

10269-IT
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITIH4

Inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4), also known as plasma kallikrein sensitive glycoprotein 120 (PK-120 or gp120), is a single chain protein that is synthesized mainly in liver and circulates at high levels in the plasma (1). ITIH4 can be processed by plasma kallikrein to 35kDa and 70 kDa fragments (1). ITIHs can be incorporated into inter-alpha-inhibitor complex with two HC linked to bikunin via chondroition sulfate (2,3) or associated with hyaluronan (HA) (3). Although the N-terminal 70kD fragment of ITIH4 contains a von-Willebrand type A domain and displays significant sequence similarity with other ITIHs (2,4), ITIH4 is the only member of the family with a kallikrein-sensitive domain in the c-terminus (1) and is not capable of complexing with bikunin (2,5). The unprocessed or 70kD fragment may bind to HA leading to stabilization of the extracellular matrix as for other HC (1,6). ITIH4 is up-regulated in acute inflammation (7) through interleukins (8). The 35kDa fragment is overexpressed in oestrogen-related conditions of select cancers and pregnancy (9) where increased plasma kallikrein can lead to cleavage and increased expression of pro-inflammatory cytokines while the long isoform of ITIH leads to upregulation of anti-inflammatory cytokines (10). ITIH4 is implicated as a diagnostic and prognostic marker in several diseases including hepatocellular carcinoma with NAFLD (11), acute lymphoblastic leukemia (12), colorectal adenoma (13) and Alzheimer's (14).

References

  1. Nishimura, H. et al. (1995) FEBS Lett. 357:207.
  2. Salier, JP et al. (1996) Biochem. J. 315:1.
  3. Zhuo, L. et al. (2004) J. Biol. Chem. 279:38079.
  4. Jean, L. et al. (1997) Genomics 41:139.
  5. Hashimoto, K. et al. (1996) J. Biochem. 119:577.
  6. Baranova. N.S et al. (2013) J. Biol. Chem. 288:29642.
  7. Gonzalez-Ramon, N. et al. (1995) FEBS Lett. 371:227.
  8. Daveau, M. et al. (1993) Biochem. J. 292:485.
  9. Mohamed, E. et al. (2013) Biomarker Research 1:19.
  10. Li, L. et al. (2018) EBioMedicine 37:535.
  11. Nakamura, N. et al. (2019) BMC Cancer 19:621.
  12. Guo, L. et al. (2019) Onco. Targets Ther. 12:3859.
  13. Ivancic, M.M. et al. (2019) Proc. Natl. Acad. Sci. USA 116:8471.
  14. Shi, X. et al. (2019) J. Alzheimers Dis. 68:1667.

Long Name

Inter-alpha-Trypsin Inhibitor Heavy Chain H4

Alternate Names

gp120, H4P, IHRP, ITI-HC4, ITIHL1, PK120

Entrez Gene IDs

3700 (Human); 16427 (Mouse); 54404 (Rat)

Gene Symbol

ITIH4

UniProt

AAI36394.1

Additional ITIH4 Products

Product Documents for Recombinant Human ITIH4 His-tag Protein, CF

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Product Specific Notices for Recombinant Human ITIH4 His-tag Protein, CF

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