Recombinant Human KAL1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9314-KA

R&D Systems, part of Bio-Techne
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9314-KA-050
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Key Product Details

Source

CHO

Accession #

EAW98759

Conjugate

Unconjugated

Applications

Bioactivity

View all KAL1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human KAL1 protein
Ala25-Tyr680, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25

Predicted Molecular Mass

75 kDa

SDS-PAGE

84-103 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human FGF basic 146 aa Recombinant Human FGF basic 146 aa (Catalog # 233-FB) is coated at 1.5 µg/mL, 100 μL/well, Recombinant Human KAL1 binds with an ED50 of 8-48 ng/mL.

Scientific Data Images for Recombinant Human KAL1 Protein, CF

Recombinant Human KAL1 Protein Bioactivity

Recombinant Human KAL1 Protein Bioactivity

When Recombinant Human FGF basic 146 aa (Catalog # 233-FB) is coated at 1.5 µg/mL, Recombinant Human KAL1 (Catalog # 9314-KA) binds with an ED50 of 8-48 ng/mL.

Formulation, Preparation and Storage

9314-KA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose.
Reconstitution
Reconstitute at 200 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KAL1

Anosmin-1, also known as Kal-1, is a 100 kDa secreted glycoprotein that regulates neuronal adhesion and migration. Mutations in Anosmin-1 are associated with the development of X-linked Kallman syndrome which can be accompanied by hearing loss (1). Mature human Anosmin-1 contains one WAP domain and four Fibronectin type-III domains (2). It is expressed by cerebellar Purkinje cells and deep nuclear neurons (2, 3), piriform cortex and olfactory bulb mitral cells (2, 4), optic tectum and retinal ganglion axons (2, 5, 6), and oligodendrocyte progenitor cells (OPC) (4). Anosmin-1 binds to Integrin beta 1/CD29, FGF R1, L1CAM, and complexes of FGF R1 with FGF basic and heparan sulfate proteoglycans, and it enhances signaling through FGF R1 (6-9). It mediates OPC adhesion, inhibits FGF basic-induced OPC migration, and enhances oligodendrocyte-mediated axon myelination (5, 6, 10, 11). It also cooperates with FGF basic to promote olfactory axon elongation and neurite outgrowth (3, 4, 8, 9, 12, 13). In addition, Anosmin-1 is up-regulated in glioblastoma and gastric cancer where it enhances tumor cell proliferation and migration (7, 14).

References

  1. Korsensky, L. and D. Ron (2016) Semin. Cell Dev. Biol. 53:101.
  2. Soussi-Yanicostas, N. et al. (1996) J. Cell Sci. 109:1749.
  3. Gianola, S. et al. (2009) Neuroscience 158:570.
  4. Soussi-Yanicostas, N. et al. (2002) Cell 109:217.
  5. Bribian, A. et al. (2008) Dev. Neurobiol. 68:1503.
  6. Bribian, A. et al. (2006) Mol. Cell. Neurosci. 33:2.
  7. Choy, C.T. et al. (2013) Endocr. Relat. Cancer 21:85.
  8. Diaz-Balzac, C.A. et al. (2015) Cell Rep. 11:1377.
  9. Gonzalez-Martinez, D. et al. (2004) J. Neurosci. 24:10384.
  10. Clemente, D. et al. (2011) J. Neurosci. 31:14899.
  11. Murcia-Belmonte, V. et al. (2016) Brain Struct. Funct. 221:1365.
  12. Soussi-Yanicostas, N. et al. (1998) J. Cell Sci. 111:2953.
  13. Garcia-Gonzalez, D. et al. (2016) Brain Struct. Funct. 221:239.
  14. Kanda, M. et al. (2016) Int. J. Cancer 138:721.

Long Name

Kallmann Syndrome 1 Sequence

Alternate Names

ADMLX, ANOS1, Anosmin 1, HH1, HHA, KAL, KALIG-1, KMS

Entrez Gene IDs

3730 (Human)

Gene Symbol

ANOS1

UniProt

EAW98759

Additional KAL1 Products

Product Documents for Recombinant Human KAL1 Protein, CF

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Product Specific Notices for Recombinant Human KAL1 Protein, CF

For research use only

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