Recombinant Human Kallikrein 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4104-SE

R&D Systems, part of Bio-Techne
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4104-SE-010
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Key Product Details

Source

NS0

Accession #

P20151

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Kallikrein 2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Kallikrein 2 protein
Met1-Pro261, with a C-terminal 10-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile25 & Leu124

Predicted Molecular Mass

28 kDa & 16 kDa

SDS-PAGE

34 kDa & 15-18 kDa, reducing conditions

Activity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).
The specific activity is >125 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

4104-SE
Formulation Supplied as a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Kallikrein 2

Kallikrein 2 (KLK2) is a secreted serine protease that is highly expressed in the human prostate gland (1). It is also known as the prostate-specific glandular kallikrein. The enzyme is highly specific for cleavage after arginine residues. KLK2 is able to activate the urokinase-type plasminogen activator (2). KLK2 is inhibited by serpins such as protein C inhibitor, antichymotrypsin, and plasminogen activator inhibitor 1 (3 ‑ 5). KLK2 is structurally related to KLK3, the prostate-specific antigen (PSA). Like PSA, KLK2 is considered to be a biomarker for prostate cancer (6).

References

  1. Chapelaine, P. et al. (1988) FEBS Lett. 236:205.
  2. Frenette, G. et al. (1997) Int. J. Cancer 71:897.
  3. Deperthes, D. et al. (1995) Biochim. Biophys. Acta 1245:311.
  4. Grauer, L.S. et al. (1998) J. Androl. 19:407.
  5. Mikolajczyk S.D. et al. (1999) Cancer Res. 59:3927.
  6. Sardana, G. et al. (2008) Clin. Chem. 54:1951.

Alternate Names

Klk1c2, KLK2, KLK2A2, Ton

Entrez Gene IDs

3817 (Human); 24841 (Rat); 102139474 (Cynomolgus Monkey)

Gene Symbol

KLK2

UniProt

P20151

Additional Kallikrein 2 Products

Product Documents for Recombinant Human Kallikrein 2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Kallikrein 2 Protein, CF

For research use only

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