Recombinant Human Kallikrein 4/Prostase Protein, CF

Kallikrein 4 (KLK4), also known as prostase or enamel matrix serine protease 1 (EMSP1), is a serine protease of the human tissue kallikrein gene family (1). Among normal tissues, human KLK4 is specifically expressed in the prostate (2). It is over-expressed in prostate cancer and this expression is regulated by hormones including androgens, esterogen and progesterone (3). rhKLK4 readily activates pro-KLK3/PSA and pro-urokinase type plasminogen activator (uPA), indicating it may initiate events involving PSA and uPA in either normal or abnormal processes (4). KLK4 may have additional roles such as functioning as one of the two major enamel proteases identified that process enamel matrix proteins (5). In addition to being a secreted enzyme, it is also a nuclear protein (3, 6). The deduced amino acid sequence of human KLK4 consists of a signal peptide, a short pro region and a mature/active enzyme. rhKLK4 can be activated in vitro by thermolysin, a zinc protease. The peptidase activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), a general serine protease inhibitor.