Recombinant Human Kininostatin Protein
R&D Systems, part of Bio-Techne | Catalog # 1396-KN
Key Product Details
Product Specifications
Source
E. coli-derived human Kininostatin protein
Lys438-Ser531
Lys438-Ser531
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys438
Predicted Molecular Mass
10.5 kDa
SDS-PAGE
14 kDa, reducing conditions
Activity
Measured by its ability to induce apoptosis of HUVEC human umbilical vein endothelial cells. Colman, R.W. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1427.
The ED50 for this effect is 1-7 µg/mL in the presence of 10 ng/mL of rhFGF basic.
The ED50 for this effect is 1-7 µg/mL in the presence of 10 ng/mL of rhFGF basic.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1396-KN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1396-KN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Kininostatin
High molecular weight kininogen (HK) is a secreted plasma glycoprotein consisting of six domains (D1 through D6). It binds specifically to endothelial cells and serves as a substrate of plasma kallikrein which releases bradykinin from within D4 of HK. Bradykinin is an active peptide that regulates multiple vascular processes. The resulting cleaved HK, called HKa, is a disulfide-linked dimer containing a heavy chain (D1 to D3) and a light chain (D5 and D6). HKa differs from HK in conformation and possesses new functional properties. It is a potent angiogenesis inhibitor that strongly inhibits endothelial cell proliferation and migration. HKa also induces apoptosis of endothelial cells. D5 of HKa has been shown to be the active region primarily responsible for the HKa anti-angiogenic activity and has been named kininostatin. While both HK and HKa are plasma proteins that are present in circulation, it is not known if kininostatin (D5) can be released from HKa and exist in plasma (1 - 4).
References
- Guo, Y. et al. (2002) International Immunopharmacology 2:1931.
- Zhang, J. et al. (2000) FASEB 14:2589.
- Colman, R.W. et al. (2000) Blood 95(2): 543.
- Guo, Y. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1427.
Entrez Gene IDs
3827 (Human)
Gene Symbol
KNG1
UniProt
Additional Kininostatin Products
Product Documents for Recombinant Human Kininostatin Protein
Product Specific Notices for Recombinant Human Kininostatin Protein
For research use only
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