Recombinant Human LAIR2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2665-LR
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human LAIR2 protein
Gln22-Pro152, with a C-terminal 6-His tag
Gln22-Pro152, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln22 predicted & Thr37
Predicted Molecular Mass
14.9 kDa
SDS-PAGE
20 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells.
When 5 x 104 cells/well are added to rhLAIR-2 coated plates (50 µg/mL with
100 µL/well), approximately 40%-60% will adhere after 10 minutes at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.
When 5 x 104 cells/well are added to rhLAIR-2 coated plates (50 µg/mL with
100 µL/well), approximately 40%-60% will adhere after 10 minutes at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.
Reviewed Applications
Read 1 review rated 5 using 2665-LR in the following applications:
Formulation, Preparation and Storage
2665-LR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: LAIR2
LAIR-2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR-1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains; (1-3) although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR-2 may compete with LAIR-1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C terminus, but retains the entire Ig domain (1-3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.
References
- Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
- Meyaard, L. et al. (1999) J. Immunol. 162:5800.
- Meyaard, L. et al. (1997) Immunity 7:283.
- Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.
Long Name
Leukocyte-associated Ig-like Receptor 2
Alternate Names
CD306
Gene Symbol
LAIR2
UniProt
Additional LAIR2 Products
Product Documents for Recombinant Human LAIR2 Protein, CF
Product Specific Notices for Recombinant Human LAIR2 Protein, CF
For research use only
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