Recombinant Human Latrophilin 3 Protein Fc Chimera, CF
R&D Systems, part of Bio-Techne | Catalog # 8915-LN
Key Product Details
Source
CHO
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Latrophilin 3/LPHN3 protein
| Human Latrophilin 3 (Phe20-Leu854) Accession # NP_056051 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Phe20
Predicted Molecular Mass
120 kDa
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human Latrophilin 3/LPHN3 Fc Chimera is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human FLRT3 (Catalog # 2795-FL) that produces 50% of the optimal binding response is approximately 0.07-0.35 μg/mL
When Recombinant Human Latrophilin 3/LPHN3 Fc Chimera is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human FLRT3 (Catalog # 2795-FL) that produces 50% of the optimal binding response is approximately 0.07-0.35 μg/mL
Formulation, Preparation and Storage
8915-LN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 250 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Latrophilin 3/LPHN3
N-terminal region of human Latrophilin-3 contains a SUEL-like lectin domain, an Olfactomedin-like domain, an O-glycosylated mucin-like region, a hormone receptor domain, and a GPCR proteolysis site (GPS). Within this approximately 120 kDa region, human Latrophilin-3 shares 96% aa sequence identity with mouse and rat Latrophilin-3. Alternative splicing of human Latrophilin-3 generates additional isoforms with a 13 aa insertion or premature truncation in the N-terminal ECD (3). Latrophilin-3 is expressed in the brain cortex (3). It binds to FLRT1, 2, and 3 and Tenurin 1 (4, 5). Its binding in trans to post-synaptic FLRT3 at glutamatergic synapses supports the maintenance of excitatory synapses as well as the amplitude of glutamatergic transmission (4, 5). Polymorphisms of Latrophilin-3 are associated with susceptibility to attention deficit/hyperactivity disorder (ADHD) (3, 6). Latrophilin-3 is also expressed in fetal adrenal glands and is down-regulated in the adult (7).
References
- Promel, S. et al. (2012) Cell Rep. 2:321.
- Matsushita, H. et al. (1999) FEBS Lett. 443:348.
- Arcos-Burgos, M. et al. (2010) Mol. Pschiatry 15:1053.
- O’Sullivan, M.L. et al. (2012) Neuron 73:903.
- O’Sullivan, M.L. et al. (2014) Neural Dev. 9:7.
- Franke, B. et al. (2012) Mol. Psychiatry 17:960.
- Xing, Y. et al. (2009) Mol. Cell. Endocrinol. 300:43.
Alternate Names
CIRL3, LEC3, Lectomedin-3, LPHN3
Gene Symbol
ADGRL3
UniProt
Additional Latrophilin 3/LPHN3 Products
Product Documents for Recombinant Human Latrophilin 3 Protein Fc Chimera, CF
Product Specific Notices for Recombinant Human Latrophilin 3 Protein Fc Chimera, CF
For research use only
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