Recombinant Human LECT2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9908-LC

R&D Systems, part of Bio-Techne
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9908-LC-050
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Key Product Details

Source

HEK293

Accession #

O14960

Conjugate

Unconjugated

Applications

Bioactivity

View all LECT2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LECT2 protein
Gly19-Leu151

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly19

Predicted Molecular Mass

15 kDa

SDS-PAGE

15-17 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 0.8-4.8 μg/mL.

Scientific Data Images for Recombinant Human LECT2 Protein, CF

Recombinant Human LECT2 Protein Bioactivity

Recombinant Human LECT2 Protein Bioactivity

Recombinant Human LECT2 (Catalog # 9908-LC) induces MC3T3-E1 mouse preosteoblast cell proliferation. The ED50 for this effect is 0.8-4.8 μg/mL.
Recombinant Human LECT2 Protein SDS-PAGE

Recombinant Human LECT2 Protein SDS-PAGE

2 μg/lane of Recombinant Human LECT2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 15-17 kDa and 12-14 kDa, respectively.

Formulation, Preparation and Storage

9908-LC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: LECT2

LECT2 (leukocyte cell-derived chemotaxin-2), also known as Chondromodulin-II, is a neutrophil chemotactic protein predominantly expressed in the liver (1). It was first identified in the heparin-binding components extracted from fetal bovine epiphyseal cartilage (2). Human LECT2 cDNA encodes a 151 amino acid (aa) precursor that includes an 18 aa signal sequence (3). The mature human LECT2 is a 16 kDa secreted hepatic protein that has a putative peptidase-M23 domain (3, 4). Human LECT2 shares 87% and 86% aa sequence identity with mouse and rat LECT2, respectively. LECT2 stimulates the growth and matrix formation of chondrocytes in vitro (2, 5, 6). In MC3T3-E1 cells, it promotes proliferation but inhibits alkaline phosphatase activity (5, 6). In vivo study suggested LECT2 can directly suppress the development of type II collagen antibody–induced arthritis (4). Recent studies have shown that LECT2 is an important regulator of tumor growth during hepatocellular carcinoma development and progression; it inhibits the angiogenic effect of HUVECs in vitro and in vivo (7).

References

  1. Yamagoe, S. et al. (1996) Immunol. Lett. 52:9.
  2. Hiraki, Y. et al. (1996) J. Bio. Chem. 271:22657.
  3. Slowik, V. and U. Apte (2017) Clin. Transl. Sci. 10:249.
  4. Okumura, A. et al. (2013) FEBS Lett. 587:404.
  5. Mori, Y. et al. (1997) FEBS Letters 406:310.
  6. Shukunami, C. et al. (1999) J. Biochem. 125:436.
  7. Chen, C.K. et al. (2016) Sci. Rep. 6:31398.

Long Name

Leukocyte Cell-derived Chemotaxin 2

Alternate Names

Chondromodulin II, MIM1

Entrez Gene IDs

3950 (Human); 16841 (Mouse)

Gene Symbol

LECT2

UniProt

O14960

Additional LECT2 Products

Product Documents for Recombinant Human LECT2 Protein, CF

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Product Specific Notices for Recombinant Human LECT2 Protein, CF

For research use only

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