Recombinant Human Leukotriene A4 Hydrolase/LTA4H Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4008-ZN
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Leukotriene A4 Hydrolase/LTA4H protein
Pro2-Asp611, with a C-terminal 10-His tag
Pro2-Asp611, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Pro2
Predicted Molecular Mass
70 kDa
SDS-PAGE
71 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC).
The specific activity is >70 pmol/min/µg, as measured under the described conditions.
The specific activity is >70 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4008-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Leukotriene A4 Hydrolase/LTA4H
Leukotrienes are a family of lipid mediators important in a variety of allergic and inflammatory reactions. Synthesized by leukocytes, these molecules are divided into two classes, the spasmogenic cysteinyl leukotrienes and LTB4, a classical chemoattractant (1). Encoded by the LTA4H gene, Leukotriene A4 Hydrolase catalyzes the conversion of unstable epoxide LTA4 to LTB4, which is the final and committed step in LTB4 biosynthesis. As a bifunctional zinc metalloenzyme, LTA4H also acts as an arginyl aminopeptidase (2). LTA4H is a drug target for anti-inflammation, and for cancer prevention and therapy (1, 3).
The mature chain of human LTA4H consists of 610 amino acids (residues 2-611). It is highly specific for LTA4, which also covalently modifies and inhibits the enzyme. The aminopeptidase activity is enhanced by monovalent anions (1). R&D Systems recombinant human LTA4H corresponds to the mature chain, and is characterized by its arginyl aminopeptidase activity.
References
- Haeggstrom, J.Z. (2006) J. Biol. Chem. 279:50639.
- Orning, L. et al. (1994) J. Biol. Chem. 269:11269.
- Chen, X. et al. (2004) Curr. Cancer Drug Targets 4:267.
Alternate Names
LTA4H
Gene Symbol
LTA4H
UniProt
Additional Leukotriene A4 Hydrolase/LTA4H Products
Product Documents for Recombinant Human Leukotriene A4 Hydrolase/LTA4H Protein, CF
Product Specific Notices for Recombinant Human Leukotriene A4 Hydrolase/LTA4H Protein, CF
For research use only
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