Recombinant Human LILRA2/CD85h/ILT1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9040-T4

R&D Systems, part of Bio-Techne
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9040-T4-050
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Key Product Details

Source

HEK293

Accession #

NP_001124389

Conjugate

Unconjugated

Applications

Bioactivity

View all LILRA2/CD85h/ILT1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LILRA2/CD85h/ILT1 protein
Gly24-Asn449, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

48 kDa

SDS-PAGE

70-81 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LILRA2/CD85h/ILT1 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7. (Catalog # 914-AN) binds with a typical ED50 of 150-900 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 9040-T4 in the following applications:

Scientific Data Images for Recombinant Human LILRA2/CD85h/ILT1 Protein, CF

Recombinant Human LILRA2/CD85h/ILT1 Protein Bioactivity

Recombinant Human LILRA2/CD85h/ILT1 Protein Bioactivity

When Recombinant Human LILRA2/CD85h/ILT1 (Catalog # 9040-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like 7 Protein (Catalog # 914-AN) binds with a typical ED50 of 150-900 ng/mL.

Formulation, Preparation and Storage

9040-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA2/CD85h/ILT1

LILRA2, also known as ILT1, CD85h, and LIR7, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA2 consists of a 426 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail (2). Alternative splicing generates isoforms with short deletions between the fourth Ig-like domain and the transmembrane region, and an isoform that is truncated C-terminal to the fourth Ig-like domain (3, 4). LILRA2 is expressed on monocytes, neutrophils, basophils, and eosinophils (5-7). It contains a positively charged  arginine residue in its transmembrane segment, enabling association with the signaling protein Fc epsilon RI gamma (5). Cross-linking of LILRA2 on monocytes induces the production of multiple cytokines as well as the upregulation of Fc gamma receptors (6, 7). Cross-linking also restricts monocyte differentiation into immature dendritic cells, phagocytic activity, and antigen presentation to T cells (6, 7). R&D Systems in-house testing indicates that LILRA2 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families.

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Borges, L. et al. (1997) J. Immunol. 159:5192.
  3. Jones, D.C. et al. (2009) Eur. J. Immunol. 39:3195.
  4. Mamegano, K. et al. (2008) Genes Immun. 9:214.
  5. Nakajima, H. et al. (1999) J. Immunol. 162:5.
  6. Lu, H.K. et al. (2012) PLoS One 7:e33478.
  7. Lee, D.J. et al. (2007) J. Immunol. 179:8128.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 2

Alternate Names

CD85h, ILT1, LIR7

Entrez Gene IDs

11027 (Human)

Gene Symbol

LILRA2

UniProt

NP_001124389

Additional LILRA2/CD85h/ILT1 Products

Product Documents for Recombinant Human LILRA2/CD85h/ILT1 Protein, CF

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Product Specific Notices for Recombinant Human LILRA2/CD85h/ILT1 Protein, CF

For research use only

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