Recombinant Human LRFN5 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9385-SA

R&D Systems, part of Bio-Techne
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9385-SA-050
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Key Product Details

Source

HEK293

Accession #

Q96NI6

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LRFN5 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LRFN5 protein
Human LRFN5
(Gln18-Gly527)
Accession # Q96NI6		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln18 inferred from enzymatic pyroglutamate treatment revealing Ile19

Predicted Molecular Mass

83 kDa

SDS-PAGE

90-107 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LRFN5 Fc Chimera is used at 1 μg/mL, the concentration of Recombinant Human LAR that produces 50% optimal binding response is 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human LRFN5 Fc Chimera Protein, CF

Recombinant Human LRFN5 Fc Chimera Protein Bioactivity

Recombinant Human LRFN5 Fc Chimera Protein Bioactivity

When Recombinant Human LRFN5 Fc Chimera (Catalog # 9385-SA) is used at 1 µg/mL, 100 µL/well, Recombinant Human LAR (Catalog # 9377-PF) binds with an ED50 of 1.5-9 µg/mL.

Formulation, Preparation and Storage

9385-SA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRFN5

Synaptic adhesion-like molecule 5 (SALM5; also leucine-rich repeat and fibronectin type-III domain-containing protein 5 (LRFN5)) is an approximately 90 kDa member of the SALM family of type I transmembrane glycoproteins (1). LRFNs comprise a family of synaptic adhesion molecules consisting of five members, each containing of an extracellular domain (ECD) of six leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 2).  LRFN-3 and -5 lack a C-terminal intracellular PDZ binding domain, which is conserved among LRFN-1, 2 and 4. Mature human LRFN-5 shares 99% amino acid sequence identity with mature mouse LRFNs. LRFN-5, like the other LRFNs, promotes neurite outgrowth as well as playing a role in neuroinflammation (3, 4). LRFN-5 is expressed in the brain and is capable of inducing presynaptic differentiation (5). Reduced expression of LRFN-5 has been associated with autism spectrum disorders and schizophrenia (6, 7). LAR family receptor protein tyrosine phosphatases (LAR-RPTPs) have been identified as novel ligands of LRFN-5 that mediates LRFN-5 dependent presynaptic differentiation in a splicing- dependent manner. LRFN-5 interacts directly with the Ig domain of LAR-RPTPs. The postsynaptic LRFN-5 promotes synapse development by trans-synaptically interacting with presynaptic  LAR-RPTPs which is important for the regulation of excitatory synaptic strength (8).

References

  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.
  4. Yuwen, Z. et al. (2106) Sci Adv. Apr;2(4).
  5. Choi, Y. et al. (2016) Sci Adv. Apr; 2(4).
  6. de Bruijn D.R.H. et al. (2010) Mol Syndromol 46.
  7. Xu, B. et al. (2009) PNAS 106:16746.
  8. Choi, Y. et.al. (2016) Sci Rep. 6:26676.

Long Name

Leucine-rich Repeat and Fibronectin Type III Domain Containing 5

Alternate Names

C14orf146, FIGLER8, SALM5

Entrez Gene IDs

145581 (Human); 238205 (Mouse); 314164 (Rat)

Gene Symbol

LRFN5

UniProt

Q96NI6

Additional LRFN5 Products

Product Documents for Recombinant Human LRFN5 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human LRFN5 Fc Chimera Protein, CF

For research use only

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