Recombinant Human LRIT2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8388-LR

R&D Systems, part of Bio-Techne
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8388-LR-050
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Key Product Details

Source

CHO

Accession #

A6NDA9

Conjugate

Unconjugated

Applications

Bioactivity

View all LRIT2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LRIT2 protein
Gln21-His462 (Leu220Phe) with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln21

Predicted Molecular Mass

49 kDa

SDS-PAGE

67-81 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.
The ED50 for this effect is 0.6-3 μg/mL.

Formulation, Preparation and Storage

8388-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRIT2

Leucine-rich Repeat, Immunoglobulin-like domain and Transmembrane domain-containing protein 2 (LRIT2), also known as leucine-rich repeat protein 22 (LRRC22), is a 550 amino acid (aa) type I transmembrane protein. Its extracellular domain (ECD) contains one LRRNT, LRRCT, Ig-like, and Fibronectin type-III domain, and four leucine-rich repeats (LRRs). Within the ECD, human LRIT2 shares approximately 75% aa sequence identity with the mouse and rat orthologs. The LRR is a structural motif consisting of 20 to 30 aa repeated 2 to 45 times that forms a hydrophobic horseshoe shape with a parallel beta sheet on the concave side and helical elements on the convex side (1-4). All LRRs are composed of a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif (1-4). LRR motifs mediate protein-protein interactions in a variety of proteins including tyrosine kinase receptors, hormone receptors, extracellular matrix-binding proteins, cell-adhesion molecules, and bacterial virulence factors (1, 3). Proteins containing LRRs play important roles in many biologically processes such as mammalian and neural development, cell polarization, gene expression, and apoptosis (2).

References

  1. Kobe, B. and J. Deisenhofer (1994) Trends Biochem. Sci. 19:415.
  2. Kobe, B. and A.V. Kajava (2001) Curr. Opin. Struct. Biol. 11:725.
  3. Enkhbayar, P. et al. (2004) Proteins 54:394.
  4. Bella, J. et al. (2008) Cell. Mol. Life Sci. 65:2307.

Long Name

Leucine-Rich Repeat, Immunoglobulin-Like And Transmembrane Domains 2

Alternate Names

LRRC22, PAL2

Entrez Gene IDs

340745 (Human); 239038 (Mouse)

Gene Symbol

LRIT2

UniProt

A6NDA9

Additional LRIT2 Products

Product Documents for Recombinant Human LRIT2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human LRIT2 Protein, CF

For research use only

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