Recombinant Human LRP-10 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10647-LR

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

NP_054764.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LRP-10 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LRP-10 protein
Human LRP-10
(His17-Pro438)
Accession # NP_054764.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His17

Predicted Molecular Mass

72 kDa

SDS-PAGE

84-96 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LRPAP (Catalog # 4296-LR) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human LRP-10 Fc Chimera (Catalog # 10647-LR) binds with an ED50 of 12-60 μg/mL.

Scientific Data Images for Recombinant Human LRP-10 Fc Chimera Protein, CF

Recombinant Human LRP-10 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human LRP-10 Fc Chimera (Catalog # 10647-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-96 kDa and 160-180 kDa, respectively.

Formulation, Preparation and Storage

10647-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-10

LRP-10 (Low-density lipoprotein receptor-related protein 10) is a member of the new subfamily of low-density lipoprotein receptor (LDLR) family that includes two other receptors LRP-3 and LRP-12 (1). It is called LRP-9 in the mice. This unique family is characterized by extracellular CUB domains and large cytoplasmic tails containing acidic dileucine (DXXLL) motifs (2). Mature human LRP-10 consists of a 424 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 252 aa cytoplasmic domain. The ECD contains 4 LDLR-A domains and 2 CUB domains. Within the ECD, human LRP-10 shares 90% aa sequence identity with mouse and rat LRP-10. LRP-10 is expressed in various tissues including brain and may be involved in apolipoprotein internalization (3). It is also localized in Trans-Golgi network (TGN) and endosomes (2,4). LRP-10 is also identified as a novel sorting receptor for Amyloid precursor protein (APP) and a decrease in LRP-10 function contributes to the pathogenesis of Alzheimer's disease (5).

References

  1. Battle, M. A. et al. (2003) Biochemistry. 42:7270.
  2. Boucher, R. et al. (2008) Histochem. Cell. Biol. 130:315.
  3. Sugiyama, T. et al. (2000) Biochemistry. 39:15817.
  4. Doray, B. et al. (2008) Traffic. 9:1551.
  5. Brodeur, J. et al. (2012) Mol. Neurodegener. 7:31.

Long Name

LDL Receptor-related Protein 10

Alternate Names

MST087

Entrez Gene IDs

26020 (Human); 65107 (Mouse); 305880 (Rat)

Gene Symbol

LRP10

UniProt

NP_054764.2 (Human)

Additional LRP-10 Products

Product Documents for Recombinant Human LRP-10 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human LRP-10 Fc Chimera Protein, CF

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