Recombinant Human LRRC4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9375-LR

R&D Systems, part of Bio-Techne
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9375-LR-050
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Key Product Details

Source

HEK293

Accession #

Q9HBW1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all LRRC4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LRRC4 protein
Human LRRC4
(Ala39-Lys527)
Accession # Q9HBW1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala39

Predicted Molecular Mass

81 kDa

SDS-PAGE

91-127 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Netrin‑G2a Fc Chimera (Catalog # 2744-NG) is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (Catalog # 9375-LR) binds with an ED50 = 2-12 ng/mL.

Scientific Data Images for Recombinant Human LRRC4 Fc Chimera Protein, CF

Recombinant Human LRRC4 Fc Chimera Protein Bioactivity

Recombinant Human LRRC4 Fc Chimera Protein Bioactivity

When Recombinant Mouse Netrin-G2a Fc Chimera (Catalog # 2744-NG) is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (Catalog # 9375-LR) binds with an ED50 = 2-12 ng/mL.

Formulation, Preparation and Storage

9375-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC4

LRRC4 (Leucine rich repeat/LRR-containing protein 4), also called NGL-2 (netrin-G ligand-2) or NAG14 (nasopharyngeal carcinoma-associated gene 14), is a 55 kDa (predicted) type I transmembrane protein that is a member of the NGL family of synaptic LRR adhesion molecules (1, 2). Human LRRC4 cDNA encodes 653 amino acids (aa) that include a 38 aa signal sequence, a 489 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 105 aa cytoplasmic domain. The ECD contains nine LRRs (aa 74-288), a C2 type Ig like domain (aa 354-440), and a Thr-rich segment (aa 455-526). Within the ECD, human LRRC4 shares 98% aa identity with mouse and rat, 99% aa identity with canine and bovine, and 99.6% aa identity with equine LRRC4. It also shares 54-55% aa identity with family members LRRC4C/NGL-1 and LRRC4B/NGL-3, but each recognizes different ligands (1). LRRC4 is predominantly expressed in the brain on neurons and astrocytes as a ligand for netrin-G2 in post-synaptic membranes (2-4). It is proposed to regulate the formation of excitatory synapses via recruitment of PSD-95 to the cytoplasmic domain after aggregation of LRRC4 at the surface (3, 5). It suppresses proliferation by down-regulating cell signaling pathways, resulting in altered expression of cell cycle regulating proteins and delay at the late G1 phase (1, 2, 6-8). It is thus considered a tumor suppressor protein and is often down-regulated in brain tumors, particularly gliomas (1, 2, 6). Forced expression of LRRC4 in tumor cells slows proliferation and promotes differentiation (1, 4, 9).

References

  1. Woo, J. et al. (2009) Mol. Cell. Neurosci. 42:1.
  2. Zhang, Q. et al. (2005) FEBS Lett. 579:3674.
  3. Kim, S. et al. (2006) Nat. Neurosci. 9:1294.
  4. Wu, M. et al. (2007) Acta Biochim Biophys Sin (Shanghai) 39:731.
  5. Nishimura-Akiyoshi, S. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14801.
  6. Wu, M. et al. (2006) Mol. Biol. Cell 17:3534.
  7. Wu, M. et al. (2008) J. Cell. Biochem. 103:245.
  8. Wu, M. et al. (2008) J. Cell. Physiol. 214:65.
  9. Zhang, W. et al. (2008) Genes Brain Behav. 7:385.

Long Name

Leucine-rich Repeat Containing 4

Alternate Names

BAG, NAG14

Entrez Gene IDs

64101 (Human); 192198 (Mouse); 641521 (Rat)

Gene Symbol

LRRC4

UniProt

Q9HBW1

Additional LRRC4 Products

Product Documents for Recombinant Human LRRC4 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human LRRC4 Fc Chimera Protein, CF

For research use only

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