Recombinant Human LRRN2 His-tag, CF

R&D Systems, part of Bio-Techne | Catalog # 10228-LR

R&D Systems, part of Bio-Techne
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10228-LR-050
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Key Product Details

Source

CHO

Accession #

O75325

Conjugate

Unconjugated

Applications

Bioactivity

View all LRRN2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LRRN2 protein
Ala19-Gly630, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19 & Val20

Predicted Molecular Mass

69 kDa

SDS-PAGE

73-81 kDa, under reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human LRRN2, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Scientific Data Images for Recombinant Human LRRN2 His-tag, CF

Recombinant Human LRRN2 His-tag SDS-PAGE

Recombinant Human LRRN2 His-tag SDS-PAGE

2 μg/lane of Recombinant Human LRRN2 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-81 kDa.

Formulation, Preparation and Storage

10228-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRN2

Leucine-rich repeat neuronal protein 2 (LRRN2) is a type I transmembrane protein containing leucine-rich repeats (1). Mature human LRRN-2 consists of a 612 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 62 aa cytoplasmic domain. Within the ECD, human LRRN-2 shares 92% with mouse and rat LRRN-2. LRRN-2 is expressed in neuronal tissue postsynaptic membranes (1, 2). It is involved in the development and maintenance of excitatory synapse in the vertebrate nervous system. LRRN-2 regulates surface expression of AMPA receptors and instructs the development of functional glutamate release sites (1, 2). LRRN-2 is up-regulated after NGF-induced differentiation and down-regulated after NGF depletion-induced apoptosis in mouse superior cervical ganglion cells (2).

References

  1. Lauren, J. et al. (2003) Genomics 81:411.
  2. Hamano, S. et al. (2004) Int. J. Oncol. 24:1457.

Long Name

Leucine-rich Repeat Neuronal 2

Alternate Names

FIGLER7, GAC1, LRANK1, LRRN5

Entrez Gene IDs

10446 (Human); 16980 (Mouse); 289020 (Rat)

Gene Symbol

LRRN2

UniProt

O75325

Additional LRRN2 Products

Product Documents for Recombinant Human LRRN2 His-tag, CF

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Product Specific Notices for Recombinant Human LRRN2 His-tag, CF

For research use only

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