Recombinant Human LTK Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9376-LK

R&D Systems, part of Bio-Techne
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9376-LK-050
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Key Product Details

Source

NS0

Accession #

P29376-1

Conjugate

Unconjugated

Applications

Bioactivity

View all LTK Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LTK protein
Ser20-Pro424, with a C-terminal His-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

42 kDa

SDS-PAGE

56-84 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LTK is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human FAM-150A that produces 50% of the optimal binding response is 40-240 ng/mL.

Scientific Data Images for Recombinant Human LTK Protein, CF

Recombinant Human LTK Protein Bioactivity

Recombinant Human LTK Protein Bioactivity

When Recombinant Human LTK (Catalog # 9376-LK) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human FAM-150A binds with an ED50 of 40-240 ng/mL.

Formulation, Preparation and Storage

9376-LK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LTK

LTK, also known as Leukocyte tyrosine kinase receptor and protein tyrosine kinase 1, is a type I transmembrane protein and shares a high degree of sequence similarity with kinases in the insulin receptor family (1). Alternative splicing generates several isoforms of 50-100 kDa (2). LTK contains a 408 aa extracellular domain, 25 aa transmembrane domain, and 415 aa cytoplasmic domain. The cytoplasmic region has several phosphotyrosines that interact with downstream signaling molecules (3). LTK is reported to undergo dimerand trimerization when under certain conditions. The extracellular domain of human LTK shares 75% homology with mouse LTK. It is expressed in preB lymphocytes, B lymphocytes, and other hematopoietic cells, as well as brain and placenta (48). Human LTK can bind to PLCgamma 1, PI3K, GAP and Raf1 in vivo (6). LTK promotes neurite outgrowth via PI3K/AKT and Ras/MAPK pathways (7). FAM150A and FAM150B can both bind to the extracellular domain of LTK, but only FAM150A is the specific ligand for LTK (9, 10).

References

  1. Honda, H. et al. (1999) Oncogene 18:3821.
  2. Yazaki, Y. et al. (1993) Proc Natl. Acad. Sci. U.S.A. 90:5404.
  3. Maru, Y., et al. (1990) Oncogene Res. 5:19.
  4. Ben-Neriah, Y. et al. (1988) Nature 333:672.
  5. Bernards, A. et al. (1990) EMBO J. 9:2279.
  6. Kozutsumi, H et al. (1994) Oncogene 9:2991.
  7. Yamada, S. et al. (2008) Neuroreport 19:1733.
  8. Roll, JD. et al. (2012) PLoS One 7:e31733.
  9. Reshetnyak, AV. et al. (2015) Proc Natl Acad Sci U S A. 112:15862.
  10. Guan, J, AV. et al. (2015) Elife. 4:e09811.

Long Name

Leukocyte Tyrosine Kinase Receptor

Alternate Names

TYK1

Entrez Gene IDs

4058 (Human); 17005 (Mouse); 311337 (Rat)

Gene Symbol

LTK

UniProt

P29376-1

Additional LTK Products

Product Documents for Recombinant Human LTK Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human LTK Protein, CF

For research use only

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