Recombinant Human Ly6E Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9970-L6

R&D Systems, part of Bio-Techne
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9970-L6-050
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Key Product Details

Source

HEK293

Accession #

Q16553

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Ly6E Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Ly6E protein
Human LY6E
(Leu21-Ser101)
Accession # Q16553		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu21

Predicted Molecular Mass

35 kDa

SDS-PAGE

37-47 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect 1-7.5 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 9970-L6 in the following applications:

Scientific Data Images for Recombinant Human Ly6E Fc Chimera Protein, CF

Recombinant Human Ly6E Fc Chimera Protein Bioactivity

Recombinant Human Ly6E Fc Chimera Protein Bioactivity

Recombinant Human Ly6E Fc Chimera (Catalog # 9970-L6) inhibits anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect is 1-7.5 μg/mL.
Recombinant Human Ly6E Fc Chimera Protein SDS-PAGE

Recombinant Human Ly6E Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human Ly6E Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-47 kDa and 70-90 kDa, respectively.

Formulation, Preparation and Storage

9970-L6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly6E

Ly6E (Lymphocyte antigen 6E), also known as Stem cell antigen 2 (SCA-2) or Thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 protein superfamily (1, 2). There are at least twenty different human Ly6 proteins that have been identified, ranging from 11 to 36 kDa. These are classified as either secreted, or membrane-bound proteins which have the GPI-anchor signaling sequence. Included in the GPI-anchored cell surface glycoproteins are Ly6A-I and Ly6K (2-4). Human Ly6E is synthesized as a 131 amino acid (aa) protein that includes a 20 aa signal peptide, an 81 aa Ly6E chain, and a 30 aa propeptide. Within the main chain region, human Ly6E shares 55% and 57% aa sequence identity with mouse and rat Ly6E, respectively. Ly6E is involved in cell signalling transduction, cell adhesion, immune regulation and drug resistance, and is over-expressed in human malignancies, including head and neck squamous cell carcinomas and lung, and oesophageal cancers (4-7). Moreover, Ly6E over-expresses in gastric cancer cells, and is important for cell survival, proliferation and migration, and could be a novel oncogenic protein for efficient diagnosis and treatment of gastric cancer (2). LY6E has recently been shown to promote viral entry of some flaviviruses, including West Nile virus, Dengue virus, and Zika virus (8).

References

  1. Gumley, T.P. et al. (1995) Immunol. Cell Biol. 73:277.
  2. Lv, Y. et al. (2018) Cell. Physiol. Biochem. 45:1219.
  3. Kong, H.K. and J.H. Park. (2012) BMB Rep. 45:595.
  4. Luo, L. et al. (2016) Oncotarget 7:11165.
  5. Yeom, C.J. et al. (2016) Oncotarget 7:65837.
  6. AlHossiny M. et al. (2016) Cancer Res.76:3376.
  7. Asundi J. et al. (2015) Clin Cancer Res. 21:3252.
  8. Mar K.B. et al. (2018) Nat Commun. 9:3603.

Long Name

Lymphocyte Antigen 6 Family Member E

Alternate Names

Ly-6E, RIGE, SCA2, TSA1

Entrez Gene IDs

4061 (Human); 17069 (Mouse); 362934 (Rat); 101866361 (Cynomolgus Monkey)

Gene Symbol

LY6E

UniProt

Q16553

Additional Ly6E Products

Product Documents for Recombinant Human Ly6E Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Ly6E Fc Chimera Protein, CF

For research use only

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