Recombinant Human MAG/Siglec-4a Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8940-MG

R&D Systems, part of Bio-Techne
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8940-MG-050
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Key Product Details

Source

HEK293

Accession #

P20916

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all MAG/Siglec-4a Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human MAG/Siglec-4a protein
Human MAG
(Gly20-Pro516)
Accession # P20916		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly20

Predicted Molecular Mass

81 kDa

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.
When immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose coated microplate, Recombinant Human MAG/Siglec‑4a Fc Chimera is able to significantly inhibit neurite outgrowth.

Reviewed Applications

Read 2 reviews rated 3.5 using 8940-MG in the following applications:

Formulation, Preparation and Storage

8940-MG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
500 µg/mL in PBS

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAG/Siglec-4a

Myelin-Associated Glycoprotein (MAG), also known as Siglec-4a, is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily (1). It is composed of an extracellular segment containing five Ig-like domains, a single transmembrane segment, and a cytoplasmic domain. Mature MAG exists as two isoforms, termed S-MAG (short) and L-MAG (long), due to alternative splicing of the cytoplasmic domain (1, 2). S-MAG has a predicted molecular weight of 67 kDa while L-MAG has a predicted molecular weight of 71 kDa (1, 2). Additionally, proteolytic cleavage of the extracellular domain produces a soluble MAG (3). Within shared regions in the extracellular domain, human MAG shares 95% aa sequence identity with mouse and rat MAG. MAG functions as an adhesion molecule during neural development. It preferentially binds to alpha-2,3-linked sialic acid terminal structures found on cell surface molecules (1, 4, 5). MAG is selectively expressed by myelinating oligodendrocytes and Schwann cells and plays an important role in axon-myelin stability (1, 4). Specifically, L-MAG is involved in myelination in the central nervous system (CNS) while S-MAG is the predominate isoform expressed during myelination in the peripheral nervous system (1). MAG is also reported to regulate the axon cytoskeleton and support the distribution of axon molecules at the nodes of Ranvier (1, 4). In addition, it has been identified as a major inhibitor of neurite outgrowth (1, 4, 6). However, MAG has also been reported to protect neurons from excitotoxicity (1, 7). MAG is believed to utilize the gangliosides GD1a and GT1b, the Nogo receptors NgR1 and NgR2/NgRH1, Integrin beta1/CD29, and PIR-B to mediate its effects (1, 4, 5, 8, 9). Soluble MAG, which is released from myelin in large quantities, has been identified in normal human tissues and in tissues from patients with neurological disorders (3). It is believed that this soluble MAG might contribute to the lack of CNS neuron regeneration after injury (3).

References

  1. Lopez, P.H. (2014) Adv. Neurobiol. 9:245.
  2. Salzer, J.L. et al. (1987) J. Cell Biol. 104:957.
  3. Tang, S. et al. (1997) Mol. Cell. Neurosci. 9:333.
  4. Schnaar, R.L. and P.H. Lopez (2009) J. Neurosci. Res. 87:3267.
  5. Schnaar, R.L. (2010) FEBS Lett. 584:1741.
  6. Akbik, F. et al. (2012) Exp. Neurol. 235:43.
  7. Lopez, P.H. et al. (2011) J. Neurochem. 116:900.
  8. Atwal, J.K. et al. (2008) Science 322:967.
  9. Goh, E.L. et al. (2008) Mol. Brain 1:10.

Long Name

Myelin-associated Glycoprotein

Alternate Names

Siglec-4a, Siglec4a

Entrez Gene IDs

4099 (Human); 17136 (Mouse); 29409 (Rat)

Gene Symbol

MAG

UniProt

P20916

Additional MAG/Siglec-4a Products

Product Documents for Recombinant Human MAG/Siglec-4a Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human MAG/Siglec-4a Fc Chimera Protein, CF

For research use only

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