Recombinant Human MAN2B1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9826-GH

R&D Systems, part of Bio-Techne
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9826-GH-050
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Key Product Details

Source

HEK293

Accession #

O00754-1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all MAN2B1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human MAN2B1 protein
Gly50-Gly1011, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly50

Predicted Molecular Mass

110 kDa

SDS-PAGE

109-127 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze 4-methylumbelliferyl-alpha -D-mannopyranoside.
The specific activity is >1,500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9826-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MAN2B1

The lysosomal catabolism of N-glycans on mammalian glycoproteins occurs through the sequential exoglycosidase digestion of oligosaccharides from the non-reducing terminus down to the carbohydrate-peptide core region (1). Included among the hydrolytic activities responsible for this oligosaccharide degradation is a broad specificity exo-alpha -mannosidase MAN2B1 that catalyzes the hydrolysis of alpha1,2-, alpha1,3-, and alpha1,6-mannoside linkages present in complex, hybrid, and high mannose Asn-linked glycans (2, 3, 4). Defects in MAN2B1 result in lysosomal alpha-mannosidosis that shows symptom of mental retardation, recurrent infections, impaired hearing and Hurler-like skeletal changes (5, 6).

Long Name

Mannosidase alpha, Class 2B, Member 1

Alternate Names

LAMAN, MANB, Mannosidase Alpha-B

Entrez Gene IDs

4125 (Human); 17159 (Mouse); 361378 (Rat)

Gene Symbol

MAN2B1

UniProt

O00754-1

Additional MAN2B1 Products

Product Documents for Recombinant Human MAN2B1 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Manufacturing Specifications

  1. Aronson, N.N. Jr. and Kuranda, M.J. (1989) FASEB J. 3:2615.
  2. Liao, Y.F. et al. (1996) J. Biol. Chem. 271:28348.
  3. Gotoda, Y. et al. (1989) Am. J. Hum. Genet. 63:1015.
  4. Nebes, V.L. and Schmidt, M.C. (1994) Biochem. Biophys. Res. Commun. 200:239.
  5. Nilssen, O, et al. (1997) Hum. Mol. Genet. 6:717.
  6. Yu, P. et al. (2015) Genet. Med. 17:971.

Product Specific Notices for Recombinant Human MAN2B1 Protein, CF

For research use only

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